Derivation of Trisomy 21 affected human embryonic stem cell line Genea021

The Genea021 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, carrying Trisomy 21, indicative of Down Syndrome. Following ICM outgrowth on inactivated human feeders, CGH and STR analyses demonstrated a 47, XY, +21 karyotype and male allele patte...

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Bibliographic Details
Published in:Stem cell research 2016-03, Vol.16 (2), p.401-404
Main Authors: Dumevska, Biljana, Bosman, Alexis, McKernan, Robert, Main, Heather, Schmidt, Uli, Peura, Teija
Format: Article
Language:English
Subjects:
Alleles
Animals
Blastocyst - cytology
Cell Differentiation
Cells, Cultured
Cellular Reprogramming
Comparative Genomic Hybridization
Down Syndrome - genetics
Down Syndrome - pathology
Human Embryonic Stem Cells - cytology
Human Embryonic Stem Cells - metabolism
Human Embryonic Stem Cells - transplantation
Humans
Karyotype
Male
Mice
Microscopy, Fluorescence
Mycoplasma
Teratoma - metabolism
Teratoma - pathology
Transcription Factors - genetics
Transcription Factors - metabolism
Transplantation, Heterologous
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Summary:The Genea021 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, carrying Trisomy 21, indicative of Down Syndrome. Following ICM outgrowth on inactivated human feeders, CGH and STR analyses demonstrated a 47, XY, +21 karyotype and male allele pattern. The hESC line had pluripotent cell morphology, 71% of cells expressed Nanog, 84% Oct4, 23% Tra1–60 and 95% SSEA4, gave a Pluritest Pluripotency score of 21.85, Novelty of 1.42, demonstrated Alkaline Phosphatase activity and tri-lineage teratoma formation. The cell line was negative for Mycoplasma and visible contamination.
ISSN:1873-5061
1876-7753
DOI:10.1016/j.scr.2016.02.010