Establishment of a Dual-Antigen Indirect ELISA Based on p30 and pB602L to Detect Antibodies against African Swine Fever Virus

African swine fever (ASF) is an acute, virulent, and highly fatal infectious disease caused by the African swine fever virus (ASFV). There is no effective vaccine or diagnostic method to prevent and control this disease currently, which highlights the significance of ASF early detection. In this stu...

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Published in:Viruses 2023-08, Vol.15 (9), p.1845
Main Authors: Zhou, Lei, Song, Jinxing, Wang, Mengxiang, Sun, Zhuoya, Sun, Junru, Tian, Panpan, Zhuang, Guoqing, Zhang, Angke, Wu, Yanan, Zhang, Gaiping
Format: Article
Language:English
Subjects:
African swine fever
African swine fever virus
Animal diseases
Antibodies
antibody detection
Antigens
Diagnosis
dual-antigen indirect ELISA
Enzyme-linked immunosorbent assay
Enzymes
Identification and classification
Infectious diseases
Methods
Plasmids
Proteins
Reproducibility
Serodiagnosis
serological diagnosis
Vaccines
Viral antibodies
Viruses
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Summary:African swine fever (ASF) is an acute, virulent, and highly fatal infectious disease caused by the African swine fever virus (ASFV). There is no effective vaccine or diagnostic method to prevent and control this disease currently, which highlights the significance of ASF early detection. In this study, we chose an early antigen and a late-expressed antigen to co-detect the target antibody, which not only helps in early detection but also improves accuracy and sensitivity. CP204L and B602L were successfully expressed as soluble proteins in an Escherichia coli vector system. By optimizing various conditions, a dual-antigen indirect ELISA for ASFV antibodies was established. The assay was non-cross-reactive with antibodies against the porcine reproductive and respiratory syndrome virus, classical swine fever virus, porcine circovirus type 2, and pseudorabies virus. The maximum serum dilution for detection of ASFV-positive sera was 1:1600. The intra-batch reproducibility coefficient of variation was
ISSN:1999-4915
1999-4915
DOI:10.3390/v15091845