Host-guest complexation studied by fluorescence correlation spectroscopy: adamantane-cyclodextrin inclusion

The host-guest complexation between an Alexa 488 labelled adamantane derivative and beta-cyclodextrin is studied by Fluorescence Correlation Spectroscopy (FCS). A 1:1 complex stoichiometry and a high association equilibrium constant of K = 5.2 x 10(4) M(-1) are obtained in aqueous solution at 25 deg...

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Bibliographic Details
Published in:International journal of molecular sciences 2010-01, Vol.11 (1), p.173-188
Main Authors: Granadero, Daniel, Bordello, Jorge, Pérez-Alvite, Maria Jesus, Novo, Mercedes, Al-Soufi, Wajih
Format: Article
Language:English
Subjects:
adamantane
Adamantane - chemistry
Chemistry
cyclodextrin
Cyclodextrins - chemistry
Equilibrium
fluorescence correlation spectroscopy
host-guest chemistry
Molecular Structure
Spectrometry, Fluorescence
Staining and Labeling
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Summary:The host-guest complexation between an Alexa 488 labelled adamantane derivative and beta-cyclodextrin is studied by Fluorescence Correlation Spectroscopy (FCS). A 1:1 complex stoichiometry and a high association equilibrium constant of K = 5.2 x 10(4) M(-1) are obtained in aqueous solution at 25 degrees C and pH = 6. The necessary experimental conditions are discussed. FCS proves to be an excellent method for the determination of stoichiometry and association equilibrium constant of this type of complexes, where both host and guest are nonfluorescent and which are therefore not easily amenable to standard fluorescence spectroscopic methods.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms11010173