Development of innovative methodology for determination of 6-thioguanine in whole blood erythrocytes by HPLC–PDA-based technique for medical diagnostics purposes

6-Thioguanine is an immunosuppressive drug, an analogue of guanine, applied to treat acute leukemia and inflammatory bowel disease. Excessive use of 6-thioguanine during clinical treatment may cause side effects. Moreover, providing a dose too low will be ineffective. Therefore, there is a critical...

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Bibliographic Details
Published in:Scientific reports 2023-08, Vol.13 (1), p.14172-14172, Article 14172
Main Authors: Noga, Maciej, Zakrzewski, Marcin, Wianowska, Dorota, Gnatowski, Maciej, Paprotny, Łukasz, Jurowski, Kamil
Format: Article
Language:English
Subjects:
631/1647/2196
639/638/11
692/308
692/308/53/2421
Blood
Body fluids
Chromatography
Dosage
Drug dosages
Erythrocytes
High-performance liquid chromatography
Humanities and Social Sciences
Immunosuppressive agents
Inflammatory bowel disease
Inflammatory bowel diseases
Laboratories
Leukemia
Leukocytes
Liquid chromatography
Metabolites
multidisciplinary
Nucleotides
Perchloric acid
Science
Science (multidisciplinary)
Side effects
Storage conditions
Thioguanine
Toxicity
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Summary:6-Thioguanine is an immunosuppressive drug, an analogue of guanine, applied to treat acute leukemia and inflammatory bowel disease. Excessive use of 6-thioguanine during clinical treatment may cause side effects. Moreover, providing a dose too low will be ineffective. Therefore, there is a critical need for a rapid, selective and routine approach to quantifying 6-thioguanine in body fluids to support a clinical application. A fully validated HPLC method has been developed to determine 6-thioguanine in whole blood samples using 5-bromouracil as an internal standard. 6-Thioguanine nucleotides were released from erythrocytes by perchloric acid, and then hydrolysed at 100 °C to the parent thiopurine, 6-thioguanine. The following validation parameters of the method were determined: specificity/selectivity, linearity range (479–17,118 ng/mL, R > 0.992), limits of detection (150 ng/mL) and quantification (479 ng/mL), accuracy (− 5.6 
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-023-41426-5