Renoprotective potential of exogen erythropoietin on experimental ruptured abdominal aortic aneurysm model: An animal study

• Published in: Iranian journal of basic medical sciences 2020-02, Vol.23 (2), p.271-276
• Main Authors: Altun, Gokalp, Cakiroglu, Yavuz, Pulathan, Zerrin, Yulug, Esin, Mentese, Ahmet
• Format: Article
• Language: English
• Subjects: Abdomen; abdominal; aneurysm; Aneurysms; aortic aneurysm; Aortic aneurysms; Carotid arteries; Coronary vessels; erythropoietin; Experiments; Heart surgery; Hemorrhagic shock; hypovolemic; Ischemia; ischemia-reperfusion injury; Kidneys; Laboratory animals; Medical research; Medicine; Methods; Mortality; Original; renoprotective effect; ruptured; shock; Tumor necrosis factor-TNF; Veins & arteries
• ISSN: 2008-3866; 2008-3874
• DOI: 10.22038/IJBMS.2019.36215.8626

The aim of this study is to investigate the renoprotective effect of erythropoietin (EPO) on hypovolemic shock and ischemia/reperfusion (IR) injury on kidneys as end-organs in an experimentally-created ruptured abdominal aortic aneurysm (rAAA) model. Thirty anesthetized Sprague-Dawley male rats were randomized to sham ((Sh n:6) (Sh+EPO n:6)) or shock and I/R groups ((S/IR n:9) (S/IR+EPO n:9)). Additional surgical procedure except aortic exploration was not performed on Sh and Sh+EPO groups. 60 min of shock, 60 min of ischemia, and 120 min of reperfusion were applied on S/IR and S/IR+EPO groups. In the S/IR and S/IR+EPO groups, hemorrhagic shock, lower torso ischemia, and reperfusion were created. At the end of the shock period, saline solutions were separately and equally administered to Sh and S/IR groups, whereas 2000 U/kg EPO was intraperitoneally administered to Sh+EPO and S/IR+EPO groups. At the end of the experimental study, some biochemical and histological parameters were studied in serum and kidney tissues. Biochemical parameters were all significantly increased in the S/IR group compared with the Sh group. These parameters were not statistically significantly different between S/IR+EPO and Sh+EPO groups. In histopathologic examination, EPO prevented high-grade injury. Our data indicate that EPO may have a renoprotective effect and reduce the systemic inflammatory response that resulted from shock and I/R in an experimental model of rAAA.