Screen Printed Based Impedimetric Immunosensor for Rapid Detection of Escherichia coli in Drinking Water

The development of a simple and low cost electrochemical impedance immunosensor based on screen printed gold electrode for rapid detection of in water is reported. The immunosensor is fabricated by immobilizing anti- antibodies onto a gold surface in a covalent way by the photochemical immobilizatio...

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Bibliographic Details
Published in:Sensors (Basel, Switzerland) Switzerland), 2020-01, Vol.20 (1), p.274
Main Authors: Cimafonte, Martina, Fulgione, Andrea, Gaglione, Rosa, Papaianni, Marina, Capparelli, Rosanna, Arciello, Angela, Bolletti Censi, Sergio, Borriello, Giorgia, Velotta, Raffaele, Della Ventura, Bartolomeo
Format: Article
Language:English
Subjects:
Antibodies
Antigens
Bacteria
Biosensors
Buffer solutions
Charge transfer
cyclic voltammetry
Drinking water
E coli
electrochemical impedance spectroscopy
Electrodes
escherichia coli
Gold
Immobilization
immunosensor
Immunosensors
Laboratories
Nyquist plots
photochemical immobilization technique
Potassium
Proteins
Voltammetry
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Summary:The development of a simple and low cost electrochemical impedance immunosensor based on screen printed gold electrode for rapid detection of in water is reported. The immunosensor is fabricated by immobilizing anti- antibodies onto a gold surface in a covalent way by the photochemical immobilization technique, a simple procedure able to bind antibodies upright onto gold surfaces. Impedance spectra are recorded in 0.01 M phosphate buffer solution (PBS) containing 10 mM Fe(CN) /Fe(CN) as redox probe. The Nyquist plots can be modelled with a modified Randles circuit, identifying the charge transfer resistance as the relevant parameter after the immobilization of antibodies, the blocking with BSA and the binding of . The introduction of a standard amplification procedure leads to a significant enhancement of the impedance increase, which allows one to measure in drinking water with a limit of detection of 3 × 10 CFU mL while preserving the rapidity of the method that requires only 1 h to provide a "yes/no" response. Additionally, by applying the Langmuir adsorption model, we are able to describe the change of in terms of the "effective" electrode, which is modified by the detection of the analyte whose microscopic conducting properties can be quantified.
ISSN:1424-8220
1424-8220
DOI:10.3390/s20010274