Effects of early florfenicol exposure on glutathione signaling pathway and PPAR signaling pathway in chick liver

• Published in: Ecotoxicology and environmental safety 2022-06, Vol.237, p.113529-113529, Article 113529
• Main Authors: Liu, Wei, Wang, Xiao, Liu, Ying, Fang, Siyuan, Wu, Zhanjun, Han, Chao, Shi, Wanyu, Bao, Yongzhan
• Format: Article
• Language: English
• Subjects: Animals; Chickens - metabolism; Florfenicol; Glutathione - metabolism; Lipid metabolism disorder; Liver; Oxidative Stress; Peroxisome Proliferator-Activated Receptors - genetics; Peroxisome Proliferator-Activated Receptors - metabolism; Proteome sequencing; Signal Transduction; Thiamphenicol - analogs & derivatives; Transcriptome sequencing
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2022.113529

Florfenicol (FFC) is a common antibiotic for animals. The nonstandard and excessive use of FFC can cause veterinary drug residues in animals, pollute soil and marine environment, and even threaten human health. Therefore, it is necessary to study the toxicity and side effects of FFC on animals. Our previous studies have proved that FFC can cause liver injury in chicks, but there are few in-depth studies on the mechanism of FFC causing liver injury at the level of signaling pathway in chicks. Therefore, transcriptome and proteome sequencing were performed and combined analysis was performed. Sequencing results showed that 1989 genes and 917 proteins were significantly changed in chick livers after FFC exposure. These genes and proteins are related to redox, glutathione transferase activity and lipid metabolism. There are 9 significantly different genes and 7 significantly different proteins in glutathione signaling pathway. Oxidative stress may occur in the liver of chicks through the change of activation state of glutathione signaling pathway. And there are 13 significantly different genes and 18 significantly different proteins in PPAR signaling pathway. The changes of PPAR signaling pathway may induce lipid metabolism disorder in liver. The verification results of qPCR and PRM were consistent with the sequencing results. We also detected GSH-Px, GSH, GST, TG, TC and ANDP levels in liver. These changes of biochemical indicators directly confirmed oxidative stress and lipid metabolism disorders were occurred in the livers of chicks treated by FFC. In conclusion, FFC could induce liver injury in chicks by regulating the expression levels of significantly different genes and proteins in glutathione signaling pathway and PPAR signaling pathway. [Display omitted] •Liver tissue of chick was detected by transcriptome and proteome sequencing.•The sequencing datas were verified by qPCR and PRM.•Florfenicol induced oxidative stress in chick liver.•Florfenicol induced the disorder of lipid metabolism in chick liver.•Part of the mechanism of florfenicol-induced liver injury is described.