A study on the hemocompatibility of dendronized chitosan derivatives in red blood cells

Dendrimers are hyperbranched macromolecules with well-defined topological structures and multivalent functionalization sites, but they may cause cytotoxicity due to the presence of cationic charge. Recently, we have introduced alkyne-terminated poly(amidoamine) (PAMAM) dendrons of different generati...

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Bibliographic Details
Published in:Drug design, development and therapy development and therapy, 2015-01, Vol.9 (default), p.2635-2645
Main Authors: Zhou, Yanfang, Li, Jiemei, Lu, Fang, Deng, Junjie, Zhang, Jiahua, Fang, Peijie, Peng, Xinsheng, Zhou, Shu-Feng
Format: Article
Language:English
Subjects:
Alkynes
Animals
Atomic force microscopy
Biocompatibility
Blood
Cell Line
Cell Membrane - drug effects
Cell Survival - drug effects
Chitin
Chitosan
Chitosan - analogs & derivatives
Chitosan - immunology
Cytotoxicity
Dendrimers
Dendrimers - chemistry
dendronized chitosan derivative
Deoxyribonucleic acid
Derivatives
DNA
Dose-response relationship (Biochemistry)
Drug delivery
Drug delivery systems
Drug development
Drugs
Efficiency
Erythrocyte Aggregation - drug effects
Erythrocytes
Erythrocytes - drug effects
Erythrocytes - immunology
hemocompatibility
Hemolysis
Hemolysis - drug effects
Hemostasis
Histocompatibility
In Vitro Techniques
Innovations
Investigations
Kidney - cytology
Kidney - immunology
Kidneys
Lipids
Lipids - chemistry
Lysis
Macromolecules
Mathematical morphology
Microscopy, Atomic Force
Nanoparticles
Observations
Original Research
PAMAM
Permeability
Pharmacy
Physiological aspects
Physiology
Plasmids
Polyethylene glycol
Polymers
Properties
Quantum dots
Rats
Rats, Sprague-Dawley
RBC
Sodium
Surface structure
Toxicity
Transfection
Vehicles
Viability
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Summary:Dendrimers are hyperbranched macromolecules with well-defined topological structures and multivalent functionalization sites, but they may cause cytotoxicity due to the presence of cationic charge. Recently, we have introduced alkyne-terminated poly(amidoamine) (PAMAM) dendrons of different generations (G=2,3) into chitosan to obtain dendronized chitosan derivatives [Cs-g-PAMAM (G=2,3)], which exhibited a better water solubility and enhanced plasmid DNA transfection efficiency. In this study, we attempted to examine the impact of Cs-g-PAMAM (G=2,3) at different concentrations (25 μg/mL, 50 μg/mL, and 100 μg/mL) on the morphology, surface structure, and viability of rat red blood cells (RBCs). The results showed that treatment of RBCs with Cs-g-PAMAM (G=2,3) at 50 μg/mL and 100 μg/mL induced a slightly higher hemolysis than Cs, and Cs-g-PAMAM (G=3) caused a slightly higher hemolysis than Cs-g-PAMAM (G=2), but all values were
ISSN:1177-8881
1177-8881
DOI:10.2147/DDDT.S77105