Genome-Wide Identification, Diversification, and Expression Analysis of Lectin Receptor-Like Kinase (LecRLK) Gene Family in Cucumber under Biotic Stress

• Published in: International journal of molecular sciences 2021-06, Vol.22 (12), p.6585
• Main Authors: Haider, Muhammad Salman, De Britto, Savitha, Nagaraj, Geetha, Gurulingaiah, Bhavya, Shekhar, Ravikant, Ito, Shin-Ichi, Jogaiah, Sudisha
• Format: Article
• Language: English
• Subjects: Amino acids; biotic stress; Chromosomes; Chromosomes, Plant; cucumber; Cucumbers; Cucumis sativus - enzymology; Cucumis sativus - genetics; Cucumis sativus - immunology; Erysiphe - immunology; Fluorescence; Fungi; Gene expression; Gene Expression Profiling; Genes; Genes, Plant; Genomes; Kinases; lectin receptor-like kinases; Lectins; Localization; Multigene Family; Pathogens; Phylogenetics; Phylogeny; plant growth-promoting fungi; Plant Immunity; Plant Proteins - genetics; Plant Proteins - metabolism; Powdery mildew; Protein Kinases - genetics; Protein Kinases - metabolism; Proteins; Stress, Physiological; Transcription
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms22126585

Members of the lectin receptor-like kinase (LecRLKs) family play a vital role in innate plant immunity. Few members of the LecRLKs family have been characterized in rice and , respectively. However, little literature is available about LecRLKs and their role against fungal infection in cucumber. In this study, 60 putative cucumber LecRLK (CsLecRLK) proteins were identified using genome-wide analysis and further characterized into L-type LecRLKs (24) and G-type LecRLKs (36) based on domain composition and phylogenetic analysis. These proteins were allocated to seven cucumber chromosomes and found to be involved in the expansion of the gene family. Subcellular localization of CsaLecRLK9 and CsaLecRLK12 showed green fluorescence signals in the plasma membrane of leaves. The transcriptional profiling of genes showed that L-type LecRLKs exhibited functional redundancy as compared to G-type LecRLKs. The qRT-PCR results indicated that both L- and G-type LecRLKs showed significant response against plant growth-promoting fungi (PGPF- Rifai), powdery mildew pathogen (PPM- (Castagne) V.P. Heluta), and combined (PGPF+PPM) treatments. The findings of this study contribute to a better understanding of the role of cucumber genes in response to PGPF, PPM, and PGPF+PPM treatments and lay the basis for the characterization of this important functional gene family.