Extraction and characterisation of pigment from Yanzhiguo [ Prunus napaulensis (Ser.) Steud.]

Yanzhiguo [ (Ser.) Steud] belongs to e family and is consumed as wild fruit, pulp and juice. However, its potential for extracting natural pigment has not yet been explored. Herein, the components in the fresh Yanzhiguo pulp were preliminarily analyzed by liquid chromatography coupled to mass spectr...

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Published in:PeerJ (San Francisco, CA) CA), 2023-08, Vol.11, p.e15517-e15517, Article e15517
Main Authors: Shi, Pingping, Luo, Haibo, Huang, Qiuqiu, Xu, Changliang, Tong, Xiuzi, Shen, Hui, Su, Huosheng, Pu, Hongmei, Wang, Haidan, Yu, Lijuan, Li, Hong
Format: Article
Language:English
Subjects:
Agricultural Science
Analysis
Antioxidant activity
Antioxidants - analysis
Biochemistry
Ethanol - analysis
Fruit - chemistry
Liquid chromatography
Mass spectrometry
Natural pigment
Plant Extracts - analysis
Plant Science
Prunus - chemistry
Prunus napaulensis (Ser.) Steud
Response surface methodology
Stability
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Summary:Yanzhiguo [ (Ser.) Steud] belongs to e family and is consumed as wild fruit, pulp and juice. However, its potential for extracting natural pigment has not yet been explored. Herein, the components in the fresh Yanzhiguo pulp were preliminarily analyzed by liquid chromatography coupled to mass spectrometry. And, the optimal pre-treatment conditions were established for further extraction of Yanzhiguo pigment based on the a* value. Then, by combining the data from single-factor experiments and response surface methodology, the optimal extraction process was established as: 35% EtOH, a liquid-solid ratio of 200:1 mL g , an extraction time of 65 min, and an extraction temperature of 100 °C. Moreover, it was found that the a* value and yield had high fitness except when extracted into ethanol (EtOH) with different concentrations. Meanwhile, our result demonstrated Yanzhiguo pigment had high stability in general environments with carmine (a synthetic pigment) as control, except for extreme environments such as direct (hot) sunlight, high temperature (75 °C) and strong alkaline (pH 11). Also, Yanzhiguo pigment exhibited good antioxidant activity. Our results contribute to more information on Yanzhiguo pigment and promote its application by providing efficient extraction technology.
ISSN:2167-8359
2167-8359
DOI:10.7717/peerj.15517