Morphological characterisation and diagnostics of Xiphinema non-americanum group species (Nematoda: Longidoridae) from Romania using mutiplex PCR

Summary Additional data on the occurrence and distribution of Xiphinema non- americanum group species in Romania are provided. Xiphinema diversicaudatum, X. index, X. vuittenezi and X. italiae were recovered from vineyards and cherry fruit trees; adults and juvenile stages were described and analyse...

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Bibliographic Details
Published in:Helminthologia 2013-09, Vol.50 (3), p.215-231
Main Authors: Groza, M., Lazarova, S., Costache, C., De Luca, F., Rosca, I., Fanelli, E., Peneva, V. K.
Format: Article
Language:English
Subjects:
Animal morphology
Biomedical and Life Sciences
Biomedicine
D2-D3
distribution
DNA
Ecology
Fruit trees
Fruits
Geographical distribution
ITS
juvenile stages
Life Sciences
Low level
Markers
Medical Microbiology
Microbiology
Morphology
Nematodes
Nucleotide sequence
PCR
Population studies
Populations
Primers
RFLP
Scientific Article
Species
Vineyards
Xiphinema
Zoology
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Summary:Summary Additional data on the occurrence and distribution of Xiphinema non- americanum group species in Romania are provided. Xiphinema diversicaudatum, X. index, X. vuittenezi and X. italiae were recovered from vineyards and cherry fruit trees; adults and juvenile stages were described and analysed and the morphology/variability discussed. Multiplex PCR diagnostic test using species-specific primers designed by Wang et al . (2003) yielded amplification products with expected lengths for all screened populations of these four species. Two ribosomal markers (D2-D3 28 LSU rDNA and ITS) were sequenced and ITS RFLP patterns were obtained from two X. vuittenezi populations, which have shown some morphological differences. Comparatively low level of interpopulation genetic dissimilarity (
ISSN:0440-6605
1336-9083
DOI:10.2478/s11687-013-0133-3