Gene Expression Analysis of Environmental Temperature and High-Fat Diet-Induced Changes in Mouse Supraclavicular Brown Adipose Tissue

Obesity, a dysregulation of adipose tissue, is a major health risk factor associated with many diseases. Brown adipose tissue (BAT)-mediated thermogenesis can potentially regulate energy expenditure, making it an attractive therapeutic target to combat obesity. Here, we characterize the effects of c...

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Bibliographic Details
Published in:Cells (Basel, Switzerland) Switzerland), 2021-06, Vol.10 (6), p.1370
Main Authors: Shi, Yufeng, Zhai, Honglei, John, Sharon, Shen, Yi-Ting, Ran, Yali, Hoang, Giang, Chen, Miao-Hsueh
Format: Article
Language:English
Subjects:
Adipocytes
adipose tissue
Adipose tissue (brown)
Body fat
Body temperature
Body weight gain
Clavicle
Diet
Energy
Energy expenditure
Experiments
Gene expression
High fat diet
Histology
Metabolism
Mitochondria
Neck
Nitrogen
Obesity
Proteins
Risk factors
supraclavicular brown adipose tissue
Thermogenesis
thermoneutrality
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Summary:Obesity, a dysregulation of adipose tissue, is a major health risk factor associated with many diseases. Brown adipose tissue (BAT)-mediated thermogenesis can potentially regulate energy expenditure, making it an attractive therapeutic target to combat obesity. Here, we characterize the effects of cold exposure, thermoneutrality, and high-fat diet (HFD) feeding on mouse supraclavicular BAT (scBAT) morphology and BAT-associated gene expression compared to other adipose depots, including the interscapular BAT (iBAT). scBAT was as sensitive to cold induced thermogenesis as iBAT and showed reduced thermogenic effect under thermoneutrality. While both scBAT and iBAT are sensitive to cold, the expression of genes involved in nutrient processing is different. The scBAT also showed less depot weight gain and more single-lipid adipocytes, while the expression of BAT thermogenic genes, such as Ucp1, remained similar or increased more under our HFD feeding regime at ambient and thermoneutral temperatures than iBAT. Together, these findings show that, in addition to its anatomical resemblance to human scBAT, mouse scBAT possesses thermogenic features distinct from those of other adipose depots. Lastly, this study also characterizes a previously unknown mouse deep neck BAT (dnBAT) depot that exhibits similar thermogenic characteristics as scBAT under cold exposure and thermoneutrality.
ISSN:2073-4409
2073-4409
DOI:10.3390/cells10061370