Rapid and selective mobilization of specific stem cell types after consumption of a polyphenol-rich extract from sea buckthorn berries ( Hippophae ) in healthy human subjects

• Published in: Clinical interventions in aging 2019-01, Vol.14, p.253-263
• Main Authors: Drapeau, Christian, Benson, Kathleen F, Jensen, Gitte S
• Format: Article
• Language: English
• Subjects: Acids; Adult; Aged; Altitude; Antigens, CD - analysis; Antigens, CD - classification; Antioxidants - pharmacology; Berries; Bone marrow; CD309; CD31; CD34; CD90; Cell Movement - drug effects; Diabetes; Disease prevention; Double-Blind Method; endothelial stem cells; Female; Flow Cytometry - methods; Fractures; Fruit; Fruits; Granulocytes; Healthy Volunteers; Heart attacks; Hippophae - chemistry; Horses; Humans; Male; Medicine; mesenchymal stem cells; Middle Aged; Original Research; Plant Extracts - chemistry; Plant Extracts - pharmacology; Polyphenols; Proanthocyanidins - pharmacology; Regeneration - drug effects; Regeneration - physiology; Rejuvenation - physiology; Stem cells; Stem Cells - classification; Stem Cells - immunology; Treatment Outcome; Wound healing
• ISSN: 1178-1998; 1176-9092; 1178-1998
• DOI: 10.2147/CIA.S186893

The aim of this study was to evaluate the effects of a proanthocyanidin-rich extract of sea buckthorn berry (SBB-PE) on the numbers of various types of adult stem cells in the blood circulation of healthy human subjects. A randomized, double-blind, placebo-controlled, cross-over trial was conducted in 12 healthy subjects. Blood samples were taken immediately before and at 1 and 2 hours after consuming either placebo or 500 mg SBB-PE. Whole blood was used for immunophenotyping and flow cytometry to quantify the numbers of CD45dim CD34+ CD309+ and CD45dim CD34+ CD309- stem cells, CD45- CD31+ CD309+ endothelial stem cells, and CD45- CD90+ mesenchymal stem cells. Consumption of SBB-PE was associated with a rapid and highly selective mobilization of CD45dim CD34+ CD309- progenitor stem cells, CD45- CD31+ CD309+ endothelial stem cells, and CD45- CD90+ lymphocytoid mesenchymal stem cells. In contrast, only minor effects were seen for CD45dim CD34+ CD309+ pluripotential stem cells. Consumption of SBB-PE resulted in selective mobilization of stem cell types involved in regenerative and reparative functions. These data may contribute to the understanding of the traditional uses of SBB for preventive health, regenerative health, and postponing the aging process.