OPNa Overexpression Is Associated with Matrix Calcification in Thyroid Cancer Cell Lines

Osteopontin (OPN) spliced variants (OPN-SV: OPNa, OPNb, and OPNc) are aberrantly expressed in tumors and frequently associated with cancer progression. This holds true for papillary thyroid carcinoma (PTC), which is the most common type of thyroid cancer (TC). PTC often presents with desmoplasia and...

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Published in:International journal of molecular sciences 2018-09, Vol.19 (10), p.2990
Main Authors: Ferreira, Luciana B, Lima, Raquel T, Bastos, Ana Clara Santos da Fonseca, Silva, Andreia M, Tavares, Catarina, Pestana, Ana, Rios, Elisabete, Eloy, Catarina, Sobrinho-Simões, Manuel, Gimba, Etel R P, Soares, Paula
Format: Article
Language:English
Subjects:
Calcinosis - metabolism
Calcinosis - pathology
Collagen - biosynthesis
Extracellular Matrix - metabolism
Female
Gene Silencing
Humans
Male
matrix calcification
Middle Aged
OPNa
Osteocalcin - metabolism
osteopontin
Osteopontin - genetics
Osteopontin - metabolism
papillary thyroid carcinoma
psammoma bodies
RNA, Messenger - genetics
RNA, Messenger - metabolism
Staining and Labeling
Thyroid Cancer, Papillary - metabolism
Thyroid Cancer, Papillary - pathology
Thyroid Neoplasms - metabolism
Thyroid Neoplasms - pathology
Time Factors
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Summary:Osteopontin (OPN) spliced variants (OPN-SV: OPNa, OPNb, and OPNc) are aberrantly expressed in tumors and frequently associated with cancer progression. This holds true for papillary thyroid carcinoma (PTC), which is the most common type of thyroid cancer (TC). PTC often presents with desmoplasia and dystrophic calcification, including psammoma bodies (PB). This work aimed to investigate total OPN (tOPN) and OPN-SV expression and their association with the presence of PB in the PTC classical variants (cPTC), as well as the involvement of OPN-SV in matrix calcification of TC cell lines. We found that cPTC samples presenting PB showed higher OPN expression levels. In TC cell lines, OPNa overexpression promotes higher matrix calcification and collagen synthesis when compared to that of clones overexpressing OPNb or OPNc. In response to OPN knockdown, calcification was inhibited, paralleled with the downregulation of calcification markers. In conclusion, our data evidenced that OPN expression is associated with the presence of PB in cPTC samples. Among the OPN-SV, OPNa is the main contributor to matrix calcification in tested TC cells, providing clues to a better understanding on the biology and ethiopathogenesis of the calcification process in TC cells.
ISSN:1422-0067
1422-0067
DOI:10.3390/ijms19102990