Stability Enhancement of Freeze-Dried Gelatin/Alginate Coacervates for bFGF Delivery

Chronic wound sites have elevated levels of proteolytic enzymes that negate the activity of topically applied growth factors. bFGF encapsulated in gelatin/alginate coacervates was protected from protease and showed better activity than bFGF in solution; however, its activity decreased with particle...

Full description

Saved in:
Bibliographic Details
Published in:Pharmaceutics 2022-11, Vol.14 (12), p.2548
Main Authors: Lee, JongOk, Ban, Eunmi, Park, Heejung, Kim, Aeri
Format: Article
Language:English
Subjects:
Acids
coacervates
cryoprotectants
Diabetes
diabetic foot ulcer
Dosage and administration
Drug delivery systems
Drug stability
Drugs
Enzymes
Evaluation
fibroblast growth factor
Fibroblast growth factors
Fibroblasts
freeze-drying
Growth factors
Materials
Polymers
Vehicles
Wound healing
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Chronic wound sites have elevated levels of proteolytic enzymes that negate the activity of topically applied growth factors. bFGF encapsulated in gelatin/alginate coacervates was protected from protease and showed better activity than bFGF in solution; however, its activity decreased with particle size and PDI increase after freeze-drying and rehydration. In this study, we aim to improve the stability of bFGF coacervates during freeze-drying to enable a topically applied growth factor delivery system for diabetic foot ulcer. Trehalose, mannitol, and Tween 80 at various concentrations were tested as cryoprotectant candidates. Trehalose improved the mechanical property of freeze-dried coacervates and physical properties after rehydration, resulting in stable size and PDI values. It also enhanced the bFGF activity in hyperglycemic human dermal fibroblasts with better cell viability, migration, and procollagen synthesis compared to the coacervates without trehalose. Hydrogen bonding interactions between trehalose and polymers probed by ATR-FTIR contribute to the stability of coacervates during freeze-drying. In conclusion, the freeze-dried gelatin/alginate coacervates encapsulating bFGF was effectively stabilized with trehalose, and the resulting coacervate composition is suggested as a potential therapeutic modality for chronic wounds including diabetic foot ulcer.
ISSN:1999-4923
1999-4923
DOI:10.3390/pharmaceutics14122548