RNase H1 Regulates Mitochondrial Transcription and Translation via the Degradation of 7S RNA

RNase H1 is able to recognize DNA/RNA heteroduplexes and to degrade their RNA component. As a consequence, it has been implicated in different aspects of mtDNA replication such as primer formation, primer removal, and replication termination, and significant differences have been reported between co...

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Bibliographic Details
Published in:Frontiers in genetics 2020-01, Vol.10, p.1393-1393
Main Authors: Reyes, Aurelio, Rusecka, Joanna, Tońska, Katarzyna, Zeviani, Massimo
Format: Article
Language:English
Subjects:
Genetics
mitochondria
mitochondrial disease
mtDNA
RNase H1
transcription
translation
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Summary:RNase H1 is able to recognize DNA/RNA heteroduplexes and to degrade their RNA component. As a consequence, it has been implicated in different aspects of mtDNA replication such as primer formation, primer removal, and replication termination, and significant differences have been reported between control and mutant skin fibroblasts from patients. However, neither mtDNA depletion nor the presence of deletions have been described in skin fibroblasts while still presenting signs of mitochondrial dysfunction (lower mitochondrial membrane potential, reduced oxygen consumption, slow growth in galactose). Here, we show that RNase H1 has an effect on mtDNA transcripts, most likely through the regulation of 7S RNA and other R-loops. The observed effect on both mitochondrial mRNAs and 16S rRNA results in decreased mitochondrial translation and subsequently mitochondrial dysfunction in cells carrying mutations in .
ISSN:1664-8021
1664-8021
DOI:10.3389/fgene.2019.01393