Amino acid solution mitigates hypothermia response and intestinal damage following exertional heat stroke in male mice

• Published in: Physiological reports 2023-05, Vol.11 (10), p.e15681-n/a
• Main Authors: King, Michelle A., Grosche, Astrid, Ward, Shauna M., Ward, Jermaine A., Sasidharan, Anusree, Mayer, Thomas A., Plamper, Mark L., Xu, Xiaodong, Ward, Matthew D., Clanton, Thomas L., Vidyasagar, Sadasivan
• Format: Article
• Language: English
• Subjects: Amino acids; Amino Acids - metabolism; Animals; Body weight; cytokines; Cytokines - metabolism; Digestive system; Environmental chambers; exercise; gastrointestinal; Gastrointestinal tract; Heat; Heat Stroke - prevention & control; Heatstroke; Hyperthermia; Hypothermia; Hypothermia - metabolism; Hypotheses; Inflammation; Intestinal Mucosa - metabolism; Laboratory animals; Life sciences; Male; Mice; Original; Pathophysiology; Permeability; Physiology; Rehydration; supplementation; Villus
• ISSN: 2051-817X
• DOI: 10.14814/phy2.15681

Increased gut permeability is implicated in the initiation and extent of the cytokine inflammatory response associated with exertional heat stroke (EHS). The primary objective of this study was to determine if a five amino acid oral rehydration solution (5AAS), specifically designed for the protection of the gastrointestinal lining, would prolong time to EHS, maintain gut function and dampen the systemic inflammatory response (SIR) measured during EHS recovery. Male C57/BL6J mice instrumented with radiotelemetry were gavaged with 150 μL of 5AAS or H2O, and ≈12 h later were either exposed to an EHS protocol where mice exercised in a 37.5°C environmental chamber to a self‐limiting maximum core temperature (Tc,max) or performed the exercise control (EXC) protocol (25°C). 5AAS pretreatment attenuated hypothermia depth and length (p