Myristica fragrans Extract Inhibits Platelet Desialylation and Activation to Ameliorate Sepsis-Associated Thrombocytopenia in a Murine CLP-Induced Sepsis Model

• Published in: International journal of molecular sciences 2023-05, Vol.24 (10), p.8863
• Main Authors: Jeong, Seong-Hun, Park, Ji-Young, Ryu, Young Bae, Kim, Woo Sik, Lee, In-Chul, Kim, Ju-Hong, Kim, Dohoon, Ha, Ji-Hye, Lee, Ba-Wool, Nam, Jiyoung, Cho, Kyoung-Oh, Kwon, Hyung-Jun
• Format: Article
• Language: English
• Subjects: Acids; Adenosine diphosphate; Animals; Blood Platelets - metabolism; Cecum; CLP; Cytokines; Drug development; Flow cytometry; Gene expression; Glycoproteins; Inflammation; Inflammatory response; Janus kinase 2; Lectins; Liver; Mice; Mortality; Myristica; Myristica fragrans; Neuraminidase - metabolism; Phosphorylation; Plants; platelet desialylation; Punctures - adverse effects; Sepsis; Sepsis - complications; Sepsis - drug therapy; Sepsis - metabolism; sepsis-associated thrombocytopenia; sialidase; Stat3 protein; Streptococcus infections; Thrombocytopenia; Thrombocytopenia - drug therapy; Thrombocytopenia - etiology; Thrombopoietin; Thrombosis
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms24108863

Sepsis, characterized by an uncontrolled host inflammatory response to infections, remains a leading cause of death in critically ill patients worldwide. Sepsis-associated thrombocytopenia (SAT), a common disease in patients with sepsis, is an indicator of disease severity. Therefore, alleviating SAT is an important aspect of sepsis treatment; however, platelet transfusion is the only available treatment strategy for SAT. The pathogenesis of SAT involves increased platelet desialylation and activation. In this study, we investigated the effects of ethanol extract (MF) on sepsis and SAT. Desialylation and activation of platelets treated with sialidase and adenosine diphosphate (platelet agonist) were assessed using flow cytometry. The extract inhibited platelet desialylation and activation via inhibiting bacterial sialidase activity in washed platelets. Moreover, MF improved survival and reduced organ damage and inflammation in a mouse model of cecal ligation and puncture (CLP)-induced sepsis. It also prevented platelet desialylation and activation via inhibiting circulating sialidase activity, while maintaining platelet count. Inhibition of platelet desialylation reduces hepatic Ashwell-Morell receptor-mediated platelet clearance, thereby reducing hepatic JAK2/STAT3 phosphorylation and thrombopoietin mRNA expression. This study lays a foundation for the development of plant-derived therapeutics for sepsis and SAT and provides insights into sialidase-inhibition-based sepsis treatment strategies.