Loading…

Fibroblast morphology, growth rate and gene expression in facial melasma

In addition to melanocytic hyperfunction, changes are observed in the upper dermis of melasma, and fibroblasts play a central role in collagen synthesis and pigmentation induction. To explore the morphology, growth rate, and gene expression profile of fibroblasts from the skin with melasma in compar...

Full description

Saved in:
Bibliographic Details
Published in:Anais brasileiros de dermatología 2022-09, Vol.97 (5), p.575-582
Main Authors: Espósito, Ana Cláudia Cavalcante, Brianezi, Gabrielli, Miot, Luciane Donida Bartoli, Miot, Hélio Amante
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c533t-470487e296b26505a1ab779ba8f3ffc531661e39d914a0fa8d943aaf48ed9b63
cites cdi_FETCH-LOGICAL-c533t-470487e296b26505a1ab779ba8f3ffc531661e39d914a0fa8d943aaf48ed9b63
container_end_page 582
container_issue 5
container_start_page 575
container_title Anais brasileiros de dermatología
container_volume 97
creator Espósito, Ana Cláudia Cavalcante
Brianezi, Gabrielli
Miot, Luciane Donida Bartoli
Miot, Hélio Amante
description In addition to melanocytic hyperfunction, changes are observed in the upper dermis of melasma, and fibroblasts play a central role in collagen synthesis and pigmentation induction. To explore the morphology, growth rate, and gene expression profile of fibroblasts from the skin with melasma in comparison to fibroblasts from the adjacent healthy skin. Ten women with facial melasma were biopsied (lesion and adjacent healthy skin), and the fragments were processed for fibroblast culture. Samples from five participants were seeded to evaluate growth (days 2, 5 and 8) and senescence (SA-β-gal) curves. The samples from the other participants were submitted to real-time PCR to comparatively evaluation of the expression of 39 genes. Cultured fibroblasts from melasma skin were morphologically less fusiform in appearance and on average a 34% (95% CI 4%‒63%) greater proportion of cells labeled with SA-β-gal than the fibroblasts from the adjacent skin. The cell growth rate was lower for the melasma samples after eight days (p < 0.01). TheWNT3A, EDN3, ESR2, PTG2, MMP1, and SOD2 genes were up-regulated, whereas the COL4A1, CSF2, DKK3, COL7A1, TIMP4, CCL2, and CDH11 genes were down-regulated in melasma skin fibroblasts when compared to the ones from adjacent healthy skin. Small sample size; absence of functional tests. Fibroblasts from the skin with melasma showed a lower growth rate, less fusiform morphology and greater accumulation of SA-β-gal than those from adjacent photo exposed skin. Moreover, their gene expression profile comprised factors that may contribute to upper dermis damage and sustained melanogenesis.
doi_str_mv 10.1016/j.abd.2021.09.012
format article
fullrecord <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_3d5d659204f94d3ea2f80c5a5fe4d60c</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><scielo_id>S0365_05962022000500575</scielo_id><els_id>S0365059622001210</els_id><doaj_id>oai_doaj_org_article_3d5d659204f94d3ea2f80c5a5fe4d60c</doaj_id><sourcerecordid>2691048873</sourcerecordid><originalsourceid>FETCH-LOGICAL-c533t-470487e296b26505a1ab779ba8f3ffc531661e39d914a0fa8d943aaf48ed9b63</originalsourceid><addsrcrecordid>eNp9kUtr3DAUhU1paSZpf0B3XnbRca-etigUSmgeEOii2Ytr6XpGg21NJU_S_PtqOqGQTUEgkM53dHVOVX1g0DBg-vOuwd43HDhrwDTA-KtqxTrQa9lJ9rpagdBqDcros-o85x0Al6Y1b6szoToJUvJVdXMV-hT7EfNSTzHtt3GMm6dP9SbFx2VbJ1yoxtnXG5qppt_7RDmHONdhrgd0Acd6ogJP-K56M-CY6f3zflHdX32_v7xZ3_24vr38drd2SohlLVuQXUvc6J5rBQoZ9m1reuwGMQxFw7RmJIw3TCIM2HkjBeIgO_Km1-Kiuj3Z-og7u09hwvRkIwb79yCmjcW0BDeSFV55rQwHORjpBSEfOnAK1UDSa3DFqzl5ZRdojHYXD2kus9ufx-DsMbiSLQcAVVarCvD1BOwP_UTe0bwkHF9M8fJmDlu7iQ_WSCUU58Xg47NBir8OlBc7hexoHHGmeMiWa8NKPl0ripSdpC7FnBMN_55hYI_t250t7dtj-xaMLe0X5suJoVLAQ6Bkjz-bHfmQyC0lovAf-g9enbPy</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2691048873</pqid></control><display><type>article</type><title>Fibroblast morphology, growth rate and gene expression in facial melasma</title><source>ScienceDirect</source><source>Open Access: PubMed Central</source><source>SciELO Brazil</source><creator>Espósito, Ana Cláudia Cavalcante ; Brianezi, Gabrielli ; Miot, Luciane Donida Bartoli ; Miot, Hélio Amante</creator><creatorcontrib>Espósito, Ana Cláudia Cavalcante ; Brianezi, Gabrielli ; Miot, Luciane Donida Bartoli ; Miot, Hélio Amante</creatorcontrib><description>In addition to melanocytic hyperfunction, changes are observed in the upper dermis of melasma, and fibroblasts play a central role in collagen synthesis and pigmentation induction. To explore the morphology, growth rate, and gene expression profile of fibroblasts from the skin with melasma in comparison to fibroblasts from the adjacent healthy skin. Ten women with facial melasma were biopsied (lesion and adjacent healthy skin), and the fragments were processed for fibroblast culture. Samples from five participants were seeded to evaluate growth (days 2, 5 and 8) and senescence (SA-β-gal) curves. The samples from the other participants were submitted to real-time PCR to comparatively evaluation of the expression of 39 genes. Cultured fibroblasts from melasma skin were morphologically less fusiform in appearance and on average a 34% (95% CI 4%‒63%) greater proportion of cells labeled with SA-β-gal than the fibroblasts from the adjacent skin. The cell growth rate was lower for the melasma samples after eight days (p &lt; 0.01). TheWNT3A, EDN3, ESR2, PTG2, MMP1, and SOD2 genes were up-regulated, whereas the COL4A1, CSF2, DKK3, COL7A1, TIMP4, CCL2, and CDH11 genes were down-regulated in melasma skin fibroblasts when compared to the ones from adjacent healthy skin. Small sample size; absence of functional tests. Fibroblasts from the skin with melasma showed a lower growth rate, less fusiform morphology and greater accumulation of SA-β-gal than those from adjacent photo exposed skin. Moreover, their gene expression profile comprised factors that may contribute to upper dermis damage and sustained melanogenesis.</description><identifier>ISSN: 0365-0596</identifier><identifier>ISSN: 1806-4841</identifier><identifier>EISSN: 1806-4841</identifier><identifier>DOI: 10.1016/j.abd.2021.09.012</identifier><identifier>PMID: 35840442</identifier><language>eng</language><publisher>Elsevier España, S.L.U</publisher><subject>Aging ; Collagen ; DERMATOLOGY ; disorders ; Melasma ; Original ; Pigmentation ; Pigmentation disorders ; Ultraviolet rays</subject><ispartof>Anais brasileiros de dermatología, 2022-09, Vol.97 (5), p.575-582</ispartof><rights>2022 Sociedade Brasileira de Dermatologia</rights><rights>2022 Sociedade Brasileira de Dermatologia. Published by Elsevier España, S.L.U. 2022 Sociedade Brasileira de Dermatologia</rights><rights>This work is licensed under a Creative Commons Attribution 4.0 International License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c533t-470487e296b26505a1ab779ba8f3ffc531661e39d914a0fa8d943aaf48ed9b63</citedby><cites>FETCH-LOGICAL-c533t-470487e296b26505a1ab779ba8f3ffc531661e39d914a0fa8d943aaf48ed9b63</cites><orcidid>0000-0003-3482-0296 ; 0000-0002-2388-7842 ; 0000-0002-2596-9294 ; 0000-0001-9283-2354</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9453522/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0365059622001210$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,3549,24150,27924,27925,45780,53791,53793</link.rule.ids></links><search><creatorcontrib>Espósito, Ana Cláudia Cavalcante</creatorcontrib><creatorcontrib>Brianezi, Gabrielli</creatorcontrib><creatorcontrib>Miot, Luciane Donida Bartoli</creatorcontrib><creatorcontrib>Miot, Hélio Amante</creatorcontrib><title>Fibroblast morphology, growth rate and gene expression in facial melasma</title><title>Anais brasileiros de dermatología</title><addtitle>An. Bras. Dermatol</addtitle><description>In addition to melanocytic hyperfunction, changes are observed in the upper dermis of melasma, and fibroblasts play a central role in collagen synthesis and pigmentation induction. To explore the morphology, growth rate, and gene expression profile of fibroblasts from the skin with melasma in comparison to fibroblasts from the adjacent healthy skin. Ten women with facial melasma were biopsied (lesion and adjacent healthy skin), and the fragments were processed for fibroblast culture. Samples from five participants were seeded to evaluate growth (days 2, 5 and 8) and senescence (SA-β-gal) curves. The samples from the other participants were submitted to real-time PCR to comparatively evaluation of the expression of 39 genes. Cultured fibroblasts from melasma skin were morphologically less fusiform in appearance and on average a 34% (95% CI 4%‒63%) greater proportion of cells labeled with SA-β-gal than the fibroblasts from the adjacent skin. The cell growth rate was lower for the melasma samples after eight days (p &lt; 0.01). TheWNT3A, EDN3, ESR2, PTG2, MMP1, and SOD2 genes were up-regulated, whereas the COL4A1, CSF2, DKK3, COL7A1, TIMP4, CCL2, and CDH11 genes were down-regulated in melasma skin fibroblasts when compared to the ones from adjacent healthy skin. Small sample size; absence of functional tests. Fibroblasts from the skin with melasma showed a lower growth rate, less fusiform morphology and greater accumulation of SA-β-gal than those from adjacent photo exposed skin. Moreover, their gene expression profile comprised factors that may contribute to upper dermis damage and sustained melanogenesis.</description><subject>Aging</subject><subject>Collagen</subject><subject>DERMATOLOGY</subject><subject>disorders</subject><subject>Melasma</subject><subject>Original</subject><subject>Pigmentation</subject><subject>Pigmentation disorders</subject><subject>Ultraviolet rays</subject><issn>0365-0596</issn><issn>1806-4841</issn><issn>1806-4841</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNp9kUtr3DAUhU1paSZpf0B3XnbRca-etigUSmgeEOii2Ytr6XpGg21NJU_S_PtqOqGQTUEgkM53dHVOVX1g0DBg-vOuwd43HDhrwDTA-KtqxTrQa9lJ9rpagdBqDcros-o85x0Al6Y1b6szoToJUvJVdXMV-hT7EfNSTzHtt3GMm6dP9SbFx2VbJ1yoxtnXG5qppt_7RDmHONdhrgd0Acd6ogJP-K56M-CY6f3zflHdX32_v7xZ3_24vr38drd2SohlLVuQXUvc6J5rBQoZ9m1reuwGMQxFw7RmJIw3TCIM2HkjBeIgO_Km1-Kiuj3Z-og7u09hwvRkIwb79yCmjcW0BDeSFV55rQwHORjpBSEfOnAK1UDSa3DFqzl5ZRdojHYXD2kus9ufx-DsMbiSLQcAVVarCvD1BOwP_UTe0bwkHF9M8fJmDlu7iQ_WSCUU58Xg47NBir8OlBc7hexoHHGmeMiWa8NKPl0ripSdpC7FnBMN_55hYI_t250t7dtj-xaMLe0X5suJoVLAQ6Bkjz-bHfmQyC0lovAf-g9enbPy</recordid><startdate>20220901</startdate><enddate>20220901</enddate><creator>Espósito, Ana Cláudia Cavalcante</creator><creator>Brianezi, Gabrielli</creator><creator>Miot, Luciane Donida Bartoli</creator><creator>Miot, Hélio Amante</creator><general>Elsevier España, S.L.U</general><general>Sociedade Brasileira de Dermatologia</general><scope>6I.</scope><scope>AAFTH</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><scope>GPN</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-3482-0296</orcidid><orcidid>https://orcid.org/0000-0002-2388-7842</orcidid><orcidid>https://orcid.org/0000-0002-2596-9294</orcidid><orcidid>https://orcid.org/0000-0001-9283-2354</orcidid></search><sort><creationdate>20220901</creationdate><title>Fibroblast morphology, growth rate and gene expression in facial melasma</title><author>Espósito, Ana Cláudia Cavalcante ; Brianezi, Gabrielli ; Miot, Luciane Donida Bartoli ; Miot, Hélio Amante</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c533t-470487e296b26505a1ab779ba8f3ffc531661e39d914a0fa8d943aaf48ed9b63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Aging</topic><topic>Collagen</topic><topic>DERMATOLOGY</topic><topic>disorders</topic><topic>Melasma</topic><topic>Original</topic><topic>Pigmentation</topic><topic>Pigmentation disorders</topic><topic>Ultraviolet rays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Espósito, Ana Cláudia Cavalcante</creatorcontrib><creatorcontrib>Brianezi, Gabrielli</creatorcontrib><creatorcontrib>Miot, Luciane Donida Bartoli</creatorcontrib><creatorcontrib>Miot, Hélio Amante</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>SciELO</collection><collection>Open Access: DOAJ - Directory of Open Access Journals</collection><jtitle>Anais brasileiros de dermatología</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Espósito, Ana Cláudia Cavalcante</au><au>Brianezi, Gabrielli</au><au>Miot, Luciane Donida Bartoli</au><au>Miot, Hélio Amante</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fibroblast morphology, growth rate and gene expression in facial melasma</atitle><jtitle>Anais brasileiros de dermatología</jtitle><addtitle>An. Bras. Dermatol</addtitle><date>2022-09-01</date><risdate>2022</risdate><volume>97</volume><issue>5</issue><spage>575</spage><epage>582</epage><pages>575-582</pages><issn>0365-0596</issn><issn>1806-4841</issn><eissn>1806-4841</eissn><abstract>In addition to melanocytic hyperfunction, changes are observed in the upper dermis of melasma, and fibroblasts play a central role in collagen synthesis and pigmentation induction. To explore the morphology, growth rate, and gene expression profile of fibroblasts from the skin with melasma in comparison to fibroblasts from the adjacent healthy skin. Ten women with facial melasma were biopsied (lesion and adjacent healthy skin), and the fragments were processed for fibroblast culture. Samples from five participants were seeded to evaluate growth (days 2, 5 and 8) and senescence (SA-β-gal) curves. The samples from the other participants were submitted to real-time PCR to comparatively evaluation of the expression of 39 genes. Cultured fibroblasts from melasma skin were morphologically less fusiform in appearance and on average a 34% (95% CI 4%‒63%) greater proportion of cells labeled with SA-β-gal than the fibroblasts from the adjacent skin. The cell growth rate was lower for the melasma samples after eight days (p &lt; 0.01). TheWNT3A, EDN3, ESR2, PTG2, MMP1, and SOD2 genes were up-regulated, whereas the COL4A1, CSF2, DKK3, COL7A1, TIMP4, CCL2, and CDH11 genes were down-regulated in melasma skin fibroblasts when compared to the ones from adjacent healthy skin. Small sample size; absence of functional tests. Fibroblasts from the skin with melasma showed a lower growth rate, less fusiform morphology and greater accumulation of SA-β-gal than those from adjacent photo exposed skin. Moreover, their gene expression profile comprised factors that may contribute to upper dermis damage and sustained melanogenesis.</abstract><pub>Elsevier España, S.L.U</pub><pmid>35840442</pmid><doi>10.1016/j.abd.2021.09.012</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0003-3482-0296</orcidid><orcidid>https://orcid.org/0000-0002-2388-7842</orcidid><orcidid>https://orcid.org/0000-0002-2596-9294</orcidid><orcidid>https://orcid.org/0000-0001-9283-2354</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0365-0596
ispartof Anais brasileiros de dermatología, 2022-09, Vol.97 (5), p.575-582
issn 0365-0596
1806-4841
1806-4841
language eng
recordid cdi_doaj_primary_oai_doaj_org_article_3d5d659204f94d3ea2f80c5a5fe4d60c
source ScienceDirect; Open Access: PubMed Central; SciELO Brazil
subjects Aging
Collagen
DERMATOLOGY
disorders
Melasma
Original
Pigmentation
Pigmentation disorders
Ultraviolet rays
title Fibroblast morphology, growth rate and gene expression in facial melasma
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T21%3A54%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Fibroblast%20morphology,%20growth%20rate%20and%20gene%20expression%20in%20facial%20melasma&rft.jtitle=Anais%20brasileiros%20de%20dermatolog%C3%ADa&rft.au=Esp%C3%B3sito,%20Ana%20Cl%C3%A1udia%20Cavalcante&rft.date=2022-09-01&rft.volume=97&rft.issue=5&rft.spage=575&rft.epage=582&rft.pages=575-582&rft.issn=0365-0596&rft.eissn=1806-4841&rft_id=info:doi/10.1016/j.abd.2021.09.012&rft_dat=%3Cproquest_doaj_%3E2691048873%3C/proquest_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c533t-470487e296b26505a1ab779ba8f3ffc531661e39d914a0fa8d943aaf48ed9b63%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2691048873&rft_id=info:pmid/35840442&rft_scielo_id=S0365_05962022000500575&rfr_iscdi=true