Effect of sucrose and potassium nitrate on biomass and saponin content of Talinum paniculatum Gaertn. hairy root in balloon-type bubble bioreactor

• Published in: Asian Pacific journal of tropical biomedicine 2015-12, Vol.5 (12), p.1027-1032
• Main Authors: Manuhara, Yosephine Sri Wulan, Kristanti, Alfinda Novi, Utami, Edy Setiti Wida, Yachya, Arif
• Format: Article
• Language: English
• Subjects: Balloon-type bubble bioreactor; Gaertn.; Saponin; Hairy; Hairy root; Liquid culture; paniculatum; root; Liq; Saponin; Talinum; Talinum paniculatum Gaertn
• ISSN: 2221-1691; 2588-9222
• DOI: 10.1016/j.apjtb.2015.09.009

Objective:To increase biomass and saponin production in hairy root culture of Talinum paniculatum Gaertn.(T.paniculatum)in balloon-type bubble bioreactor(BTBB).Methods:Hairy roots which were collected from leaf explants of T.paniculatum were infected by Agrobacterium rhizogenes strain LB510.The hairy roots were cultivated at400 m L Murashige and Skoog liquid medium without growth regulator(MS0)in1 000 m L BTBB.Each BTBB had 2 g hairy roots as initial inoculum and these cultures were treated with various concentrations of sucrose(3%,4%,5%,6%w/v)and potassium nitrate(0.5,1.0,1.5 and 2.0 strength of MS medium).Cultures were maintained for 14days.Fresh and dry weights of hairy roots at the end of culture were investigated.Results:Various concentrations of sucrose influenced the biomass accumulation of hairy roots.Maximum biomass was reached by MS medium supplemented with 6%sucrose and it was approximately threefold higher than control.Culture supplemented with potassium nitrate at 2.0 strength of MS0 could increase biomass accumulation of hairy roots until 0.14 g dry weight and it was almost threefold higher than control.However,the maximum saponin content was obtained by MS medium supplemented with 5%sucrose and 2.0 strength potassium nitrate of MS.Conclusions:Based on this research,those conditions can be used to produce biomass and saponin of hairy root of T.paniculatum in the large scale.