Correlated flickering of erythrocytes membrane observed with dual time resolved membrane fluctuation spectroscopy under different d-glucose concentrations

A correlated human red blood cell membrane fluctuation dependent on d -glucose concentration was found with dual time resolved membrane fluctuation spectroscopy (D-TRMFS). This new technique is a modified version of the dual optical tweezers method that has been adapted to measure the mechanical pro...

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Published in:Scientific reports 2021-01, Vol.11 (1), p.2429-12, Article 2429
Main Authors: Tapia, J., Vera, N., Aguilar, Joao, González, M., Sánchez, S. A., Coelho, P., Saavedra, C., Staforelli, J.
Format: Article
Language:English
Subjects:
2-Naphthylamine - analogs & derivatives
2-Naphthylamine - pharmacology
631/57
631/57/2270
631/80
639/624
639/624/1107
Adult
Biomechanical Phenomena
Cell membranes
Correlation analysis
Erythrocyte Membrane - drug effects
Erythrocyte Membrane - physiology
Erythrocytes
Fluidity
Glucose
Glucose - pharmacology
Humanities and Social Sciences
Humans
Laurates - pharmacology
Lipid bilayers
Mechanical properties
Membrane fluidity
Membrane Fluidity - drug effects
Membranes
multidisciplinary
Science
Science (multidisciplinary)
Signal Processing, Computer-Assisted
Spectroscopy
Spectrum Analysis
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cited_by cdi_FETCH-LOGICAL-c540t-9570259b5a02598b49f44a6d3c5b92526e380c56f569aaca850238b706de896c3
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container_title Scientific reports
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creator Tapia, J.
Vera, N.
Aguilar, Joao
González, M.
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Coelho, P.
Saavedra, C.
Staforelli, J.
description A correlated human red blood cell membrane fluctuation dependent on d -glucose concentration was found with dual time resolved membrane fluctuation spectroscopy (D-TRMFS). This new technique is a modified version of the dual optical tweezers method that has been adapted to measure the mechanical properties of red blood cells (RBCs) at distant membrane points simultaneously, enabling correlation analysis. Mechanical parameters under different d -glucose concentrations were obtained from direct membrane flickering measurements, complemented with membrane fluidity measurements using Laurdan Generalized Polarization (GP) Microscopy. Our results show an increase in the fluctuation amplitude of the lipid bilayer, and a decline in tension value, bending modulus and fluidity as d -glucose concentration increases. Metabolic mechanisms are proposed as explanations for the results.
doi_str_mv 10.1038/s41598-021-82018-5
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source PubMed (Medline); Full-Text Journals in Chemistry (Open access); Publicly Available Content (ProQuest); Springer Nature - nature.com Journals - Fully Open Access
subjects 2-Naphthylamine - analogs & derivatives
2-Naphthylamine - pharmacology
631/57
631/57/2270
631/80
639/624
639/624/1107
Adult
Biomechanical Phenomena
Cell membranes
Correlation analysis
Erythrocyte Membrane - drug effects
Erythrocyte Membrane - physiology
Erythrocytes
Fluidity
Glucose
Glucose - pharmacology
Humanities and Social Sciences
Humans
Laurates - pharmacology
Lipid bilayers
Mechanical properties
Membrane fluidity
Membrane Fluidity - drug effects
Membranes
multidisciplinary
Science
Science (multidisciplinary)
Signal Processing, Computer-Assisted
Spectroscopy
Spectrum Analysis
title Correlated flickering of erythrocytes membrane observed with dual time resolved membrane fluctuation spectroscopy under different d-glucose concentrations
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