A Novel Aptamer-Imprinted Polymer-Based Electrochemical Biosensor for the Detection of Lead in Aquatic Products

Lead contamination in aquatic products is one of the main hazard factors. The aptasensor is a promising detection method for lead ion (Pb(II)) because of its selectivity, but it is easily affected by pH. The combination of ion-imprinted polymers(IIP) with aptamers may improve their stability in diff...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2022-12, Vol.28 (1), p.196
Main Authors: Zhu, Nianxin, Liu, Xinna, Peng, Kaimin, Cao, Hui, Yuan, Min, Ye, Tai, Wu, Xiuxiu, Yin, Fengqin, Yu, Jinsong, Hao, Liling, Xu, Fei
Format: Article
Language:English
Subjects:
aptamer-imprinted polymer
Aptamers
Biosensors
Book publishing
Chitosan
chitosan–graphene oxide composite
Conformation
Contamination
Crosslinked polymers
Detectors
Electric properties
Electrochemistry
Electrodes
Ethylenediaminetetraacetic acid
Glassy carbon
Graphene
Imprinted polymers
Lead
lead detection
Medical research
Metal ions
Nanoparticles
Polymer industry
Polymers
Scientific imaging
Selectivity
Shellfish
Stability
Voltammetry
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Summary:Lead contamination in aquatic products is one of the main hazard factors. The aptasensor is a promising detection method for lead ion (Pb(II)) because of its selectivity, but it is easily affected by pH. The combination of ion-imprinted polymers(IIP) with aptamers may improve their stability in different pH conditions. This paper developed a novel electrochemical biosensor for Pb(II) detection by using aptamer-imprinted polymer as a recognition element. The glassy carbon electrode was modified with gold nanoparticles and aptamers. After the aptamer was induced by Pb(II) to form a G-quadruplex conformation, a chitosan-graphene oxide was electrodeposited and cross-linked with glutaraldehyde to form an imprint layer, improving the stability of the biosensor. Under the optimal experimental conditions, the current signal change (∆I) showed a linear correlation of the content of Pb(II) in the range of 0.1-2.0 μg/mL with a detection limit of 0.0796 μg/mL (S/N = 3). The biosensor also exhibited high selectivity for the determination of Pb(II) in the presence of other interfering metal ion. At the same time, the stability of the imprinted layer made the sensor applicable to the detection environment with a pH of 6.4-8.0. Moreover, the sensor was successfully applied to the detection of Pb(II) in mantis shrimp.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules28010196