The cytotoxicity effect and identification of bioactive compounds of Prismatomeris glabra crude leaf extracts against breast cancer cells

• Published in: Beni-Suef University journal of basic and applied sciences 2024-12, Vol.13 (1), p.33-18, Article 33
• Main Authors: Zulkipli, Ninie Nadia, Rahman, Sholehah Ab, Taib, Wan Rohani Wan, Razali, Razifah Mohd, Ismail, Illyana, Ahmad, Wan Amir Nizam Wan, Daud, Che Ku Dahlan Che Ku
• Format: Article
• Language: English
• Subjects: Anemia; Anticancer properties; Antineoplastic drugs; Bioactive compounds; Biocompatibility; Biological activity; Breast cancer; Cancer therapies; Cell culture; Cell death; Cell morphology; Cytotoxicity; Drug development; Drugs; Ethanol; Flavonoids; Geographical distribution; Hepatitis; Herbal medicine; Leaves; Liquid chromatography–mass spectrometry (LC–MS); MCF-7 cell line; Medicine; Medicine & Public Health; MTT assay; Plant extracts; Prismatomeris glabra; Toxicity; Triterpenoids
• ISSN: 2314-8543; 2314-8535; 2314-8543
• DOI: 10.1186/s43088-024-00490-0

Background Despite the fact that natives of Southeast Asia have been consuming Prismatomeris glabra for decades for a variety of health benefits, research on this species is not as extensive as that on other species due to its limited distribution. The purpose of this study was to determine the cytotoxicity and identify the bioactive compounds of P. glabra crude leaf extracts against the MCF-7 cell line. Results We first examined the potential cytotoxic activity of P. glabra using the MTT assay against the MCF-7 cell line to determine the IC 50 of the plant extracts at various concentrations at three time points (24 h, 48 h, and 72 h). Across all time points, the MTT assay revealed that the aqueous extract exhibited the lowest IC 50 values ( p