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The cytotoxicity effect and identification of bioactive compounds of Prismatomeris glabra crude leaf extracts against breast cancer cells
Background Despite the fact that natives of Southeast Asia have been consuming Prismatomeris glabra for decades for a variety of health benefits, research on this species is not as extensive as that on other species due to its limited distribution. The purpose of this study was to determine the cyto...
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| Published in: | Beni-Suef University journal of basic and applied sciences 2024-12, Vol.13 (1), p.33-18, Article 33 |
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| container_title | Beni-Suef University journal of basic and applied sciences |
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| creator | Zulkipli, Ninie Nadia Rahman, Sholehah Ab Taib, Wan Rohani Wan Razali, Razifah Mohd Ismail, Illyana Ahmad, Wan Amir Nizam Wan Daud, Che Ku Dahlan Che Ku |
| description | Background
Despite the fact that natives of Southeast Asia have been consuming
Prismatomeris glabra
for decades for a variety of health benefits, research on this species is not as extensive as that on other species due to its limited distribution. The purpose of this study was to determine the cytotoxicity and identify the bioactive compounds of
P. glabra
crude leaf extracts against the MCF-7 cell line.
Results
We first examined the potential cytotoxic activity of
P. glabra
using the MTT assay against the MCF-7 cell line to determine the IC
50
of the plant extracts at various concentrations at three time points (24 h, 48 h, and 72 h). Across all time points, the MTT assay revealed that the aqueous extract exhibited the lowest IC
50
values (
p
|
| doi_str_mv | 10.1186/s43088-024-00490-0 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_3dfb741a12fe46aa92a34d3b7b770953</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_3dfb741a12fe46aa92a34d3b7b770953</doaj_id><sourcerecordid>3028042817</sourcerecordid><originalsourceid>FETCH-LOGICAL-c447t-2c3b9fc751719cb85529d82c19ae6d8ee72d8d55e6b4d168305815ff309a6ed53</originalsourceid><addsrcrecordid>eNp9kc1uFDEQhEcIJKKQF-BkifOAf2fsI4r4iRQpHMLZatvtxavZ8WJ7UfYReGucDAJOnLrUqq-6pRqG14y-ZUxP76oUVOuRcjlSKg0d6bPhggsmR62keP6Pfjlc1bqnlDLDKePiYvh5_w2JP7fc8kPyqZ0Jxoi-EVgDSQHXlmLy0FJeSY7EpQy-pR-dyYdjPq2hPq6_lFQP0PIBuyC7BVwB4sspIFkQIsGHVjpXCewgrbURVxD68LB6LMTjstRXw4sIS8Wr3_Ny-Prxw_315_H27tPN9fvb0Us5t5F74Uz0s2IzM95ppbgJmntmAKegEWcedFAKJycDm7SgSjMVo6AGJgxKXA43W27IsLfHkg5QzjZDsk-LXHYWSkt-QStCdLNkwHhEOQEYDkIG4WY3z9Qo0bPebFnHkr-fsDa7z6ey9vetoFxTyTWbu4tvLl9yrQXjn6uM2scG7dag7Q3apwYt7ZDYoNrN6w7L3-j_UL8ANJ6gXQ</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3028042817</pqid></control><display><type>article</type><title>The cytotoxicity effect and identification of bioactive compounds of Prismatomeris glabra crude leaf extracts against breast cancer cells</title><source>Publicly Available Content Database</source><source>Springer Nature - SpringerLink Journals - Fully Open Access </source><creator>Zulkipli, Ninie Nadia ; Rahman, Sholehah Ab ; Taib, Wan Rohani Wan ; Razali, Razifah Mohd ; Ismail, Illyana ; Ahmad, Wan Amir Nizam Wan ; Daud, Che Ku Dahlan Che Ku</creator><creatorcontrib>Zulkipli, Ninie Nadia ; Rahman, Sholehah Ab ; Taib, Wan Rohani Wan ; Razali, Razifah Mohd ; Ismail, Illyana ; Ahmad, Wan Amir Nizam Wan ; Daud, Che Ku Dahlan Che Ku</creatorcontrib><description>Background
Despite the fact that natives of Southeast Asia have been consuming
Prismatomeris glabra
for decades for a variety of health benefits, research on this species is not as extensive as that on other species due to its limited distribution. The purpose of this study was to determine the cytotoxicity and identify the bioactive compounds of
P. glabra
crude leaf extracts against the MCF-7 cell line.
Results
We first examined the potential cytotoxic activity of
P. glabra
using the MTT assay against the MCF-7 cell line to determine the IC
50
of the plant extracts at various concentrations at three time points (24 h, 48 h, and 72 h). Across all time points, the MTT assay revealed that the aqueous extract exhibited the lowest IC
50
values (
p
< 0.05) compared to the ethanol and methanol extracts. All plant extracts exerted the ability to induce cell death in the MCF-7 cell line at all time points, and the optimal time for
P. glabra
to manifest its antiproliferative activities and promote cell death was 48 h. LC–MS analysis was conducted to reveal the components in plant extracts. Forty compounds were discovered in P
. glabra
's extracts, with the majority being flavonoids and triterpenoids. Five similar compounds were present in all three extracts. Further research should be conducted on these compounds to unveil a compound that fulfils the criteria as a promising anticancer agent. This research is of the utmost importance, as it provides a fundamental framework for the identification of alternative therapies for breast cancer and contributes implicitly to the development of new drugs.
Conclusions
This study discovered that
P. glabra
crude leaf extracts have the potential to inhibit the MCF-7 cell line by inducing cell death.</description><identifier>ISSN: 2314-8543</identifier><identifier>ISSN: 2314-8535</identifier><identifier>EISSN: 2314-8543</identifier><identifier>DOI: 10.1186/s43088-024-00490-0</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Anemia ; Anticancer properties ; Antineoplastic drugs ; Bioactive compounds ; Biocompatibility ; Biological activity ; Breast cancer ; Cancer therapies ; Cell culture ; Cell death ; Cell morphology ; Cytotoxicity ; Drug development ; Drugs ; Ethanol ; Flavonoids ; Geographical distribution ; Hepatitis ; Herbal medicine ; Leaves ; Liquid chromatography–mass spectrometry (LC–MS) ; MCF-7 cell line ; Medicine ; Medicine & Public Health ; MTT assay ; Plant extracts ; Prismatomeris glabra ; Toxicity ; Triterpenoids</subject><ispartof>Beni-Suef University journal of basic and applied sciences, 2024-12, Vol.13 (1), p.33-18, Article 33</ispartof><rights>The Author(s) 2024</rights><rights>The Author(s) 2024. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c447t-2c3b9fc751719cb85529d82c19ae6d8ee72d8d55e6b4d168305815ff309a6ed53</cites><orcidid>0000-0001-7074-1144 ; 0000-0002-0710-2603 ; 0000-0002-5613-4919 ; 0000-0001-9455-5768 ; 0000-0002-0771-2559</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/3028042817/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/3028042817?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,25753,27924,27925,37012,44590,75126</link.rule.ids></links><search><creatorcontrib>Zulkipli, Ninie Nadia</creatorcontrib><creatorcontrib>Rahman, Sholehah Ab</creatorcontrib><creatorcontrib>Taib, Wan Rohani Wan</creatorcontrib><creatorcontrib>Razali, Razifah Mohd</creatorcontrib><creatorcontrib>Ismail, Illyana</creatorcontrib><creatorcontrib>Ahmad, Wan Amir Nizam Wan</creatorcontrib><creatorcontrib>Daud, Che Ku Dahlan Che Ku</creatorcontrib><title>The cytotoxicity effect and identification of bioactive compounds of Prismatomeris glabra crude leaf extracts against breast cancer cells</title><title>Beni-Suef University journal of basic and applied sciences</title><addtitle>Beni-Suef Univ J Basic Appl Sci</addtitle><description>Background
Despite the fact that natives of Southeast Asia have been consuming
Prismatomeris glabra
for decades for a variety of health benefits, research on this species is not as extensive as that on other species due to its limited distribution. The purpose of this study was to determine the cytotoxicity and identify the bioactive compounds of
P. glabra
crude leaf extracts against the MCF-7 cell line.
Results
We first examined the potential cytotoxic activity of
P. glabra
using the MTT assay against the MCF-7 cell line to determine the IC
50
of the plant extracts at various concentrations at three time points (24 h, 48 h, and 72 h). Across all time points, the MTT assay revealed that the aqueous extract exhibited the lowest IC
50
values (
p
< 0.05) compared to the ethanol and methanol extracts. All plant extracts exerted the ability to induce cell death in the MCF-7 cell line at all time points, and the optimal time for
P. glabra
to manifest its antiproliferative activities and promote cell death was 48 h. LC–MS analysis was conducted to reveal the components in plant extracts. Forty compounds were discovered in P
. glabra
's extracts, with the majority being flavonoids and triterpenoids. Five similar compounds were present in all three extracts. Further research should be conducted on these compounds to unveil a compound that fulfils the criteria as a promising anticancer agent. This research is of the utmost importance, as it provides a fundamental framework for the identification of alternative therapies for breast cancer and contributes implicitly to the development of new drugs.
Conclusions
This study discovered that
P. glabra
crude leaf extracts have the potential to inhibit the MCF-7 cell line by inducing cell death.</description><subject>Anemia</subject><subject>Anticancer properties</subject><subject>Antineoplastic drugs</subject><subject>Bioactive compounds</subject><subject>Biocompatibility</subject><subject>Biological activity</subject><subject>Breast cancer</subject><subject>Cancer therapies</subject><subject>Cell culture</subject><subject>Cell death</subject><subject>Cell morphology</subject><subject>Cytotoxicity</subject><subject>Drug development</subject><subject>Drugs</subject><subject>Ethanol</subject><subject>Flavonoids</subject><subject>Geographical distribution</subject><subject>Hepatitis</subject><subject>Herbal medicine</subject><subject>Leaves</subject><subject>Liquid chromatography–mass spectrometry (LC–MS)</subject><subject>MCF-7 cell line</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>MTT assay</subject><subject>Plant extracts</subject><subject>Prismatomeris glabra</subject><subject>Toxicity</subject><subject>Triterpenoids</subject><issn>2314-8543</issn><issn>2314-8535</issn><issn>2314-8543</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNp9kc1uFDEQhEcIJKKQF-BkifOAf2fsI4r4iRQpHMLZatvtxavZ8WJ7UfYReGucDAJOnLrUqq-6pRqG14y-ZUxP76oUVOuRcjlSKg0d6bPhggsmR62keP6Pfjlc1bqnlDLDKePiYvh5_w2JP7fc8kPyqZ0Jxoi-EVgDSQHXlmLy0FJeSY7EpQy-pR-dyYdjPq2hPq6_lFQP0PIBuyC7BVwB4sspIFkQIsGHVjpXCewgrbURVxD68LB6LMTjstRXw4sIS8Wr3_Ny-Prxw_315_H27tPN9fvb0Us5t5F74Uz0s2IzM95ppbgJmntmAKegEWcedFAKJycDm7SgSjMVo6AGJgxKXA43W27IsLfHkg5QzjZDsk-LXHYWSkt-QStCdLNkwHhEOQEYDkIG4WY3z9Qo0bPebFnHkr-fsDa7z6ey9vetoFxTyTWbu4tvLl9yrQXjn6uM2scG7dag7Q3apwYt7ZDYoNrN6w7L3-j_UL8ANJ6gXQ</recordid><startdate>20241201</startdate><enddate>20241201</enddate><creator>Zulkipli, Ninie Nadia</creator><creator>Rahman, Sholehah Ab</creator><creator>Taib, Wan Rohani Wan</creator><creator>Razali, Razifah Mohd</creator><creator>Ismail, Illyana</creator><creator>Ahmad, Wan Amir Nizam Wan</creator><creator>Daud, Che Ku Dahlan Che Ku</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><general>SpringerOpen</general><scope>C6C</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>L6V</scope><scope>LK8</scope><scope>M7P</scope><scope>M7S</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0001-7074-1144</orcidid><orcidid>https://orcid.org/0000-0002-0710-2603</orcidid><orcidid>https://orcid.org/0000-0002-5613-4919</orcidid><orcidid>https://orcid.org/0000-0001-9455-5768</orcidid><orcidid>https://orcid.org/0000-0002-0771-2559</orcidid></search><sort><creationdate>20241201</creationdate><title>The cytotoxicity effect and identification of bioactive compounds of Prismatomeris glabra crude leaf extracts against breast cancer cells</title><author>Zulkipli, Ninie Nadia ; Rahman, Sholehah Ab ; Taib, Wan Rohani Wan ; Razali, Razifah Mohd ; Ismail, Illyana ; Ahmad, Wan Amir Nizam Wan ; Daud, Che Ku Dahlan Che Ku</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c447t-2c3b9fc751719cb85529d82c19ae6d8ee72d8d55e6b4d168305815ff309a6ed53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Anemia</topic><topic>Anticancer properties</topic><topic>Antineoplastic drugs</topic><topic>Bioactive compounds</topic><topic>Biocompatibility</topic><topic>Biological activity</topic><topic>Breast cancer</topic><topic>Cancer therapies</topic><topic>Cell culture</topic><topic>Cell death</topic><topic>Cell morphology</topic><topic>Cytotoxicity</topic><topic>Drug development</topic><topic>Drugs</topic><topic>Ethanol</topic><topic>Flavonoids</topic><topic>Geographical distribution</topic><topic>Hepatitis</topic><topic>Herbal medicine</topic><topic>Leaves</topic><topic>Liquid chromatography–mass spectrometry (LC–MS)</topic><topic>MCF-7 cell line</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>MTT assay</topic><topic>Plant extracts</topic><topic>Prismatomeris glabra</topic><topic>Toxicity</topic><topic>Triterpenoids</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zulkipli, Ninie Nadia</creatorcontrib><creatorcontrib>Rahman, Sholehah Ab</creatorcontrib><creatorcontrib>Taib, Wan Rohani Wan</creatorcontrib><creatorcontrib>Razali, Razifah Mohd</creatorcontrib><creatorcontrib>Ismail, Illyana</creatorcontrib><creatorcontrib>Ahmad, Wan Amir Nizam Wan</creatorcontrib><creatorcontrib>Daud, Che Ku Dahlan Che Ku</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Beni-Suef University journal of basic and applied sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zulkipli, Ninie Nadia</au><au>Rahman, Sholehah Ab</au><au>Taib, Wan Rohani Wan</au><au>Razali, Razifah Mohd</au><au>Ismail, Illyana</au><au>Ahmad, Wan Amir Nizam Wan</au><au>Daud, Che Ku Dahlan Che Ku</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The cytotoxicity effect and identification of bioactive compounds of Prismatomeris glabra crude leaf extracts against breast cancer cells</atitle><jtitle>Beni-Suef University journal of basic and applied sciences</jtitle><stitle>Beni-Suef Univ J Basic Appl Sci</stitle><date>2024-12-01</date><risdate>2024</risdate><volume>13</volume><issue>1</issue><spage>33</spage><epage>18</epage><pages>33-18</pages><artnum>33</artnum><issn>2314-8543</issn><issn>2314-8535</issn><eissn>2314-8543</eissn><abstract>Background
Despite the fact that natives of Southeast Asia have been consuming
Prismatomeris glabra
for decades for a variety of health benefits, research on this species is not as extensive as that on other species due to its limited distribution. The purpose of this study was to determine the cytotoxicity and identify the bioactive compounds of
P. glabra
crude leaf extracts against the MCF-7 cell line.
Results
We first examined the potential cytotoxic activity of
P. glabra
using the MTT assay against the MCF-7 cell line to determine the IC
50
of the plant extracts at various concentrations at three time points (24 h, 48 h, and 72 h). Across all time points, the MTT assay revealed that the aqueous extract exhibited the lowest IC
50
values (
p
< 0.05) compared to the ethanol and methanol extracts. All plant extracts exerted the ability to induce cell death in the MCF-7 cell line at all time points, and the optimal time for
P. glabra
to manifest its antiproliferative activities and promote cell death was 48 h. LC–MS analysis was conducted to reveal the components in plant extracts. Forty compounds were discovered in P
. glabra
's extracts, with the majority being flavonoids and triterpenoids. Five similar compounds were present in all three extracts. Further research should be conducted on these compounds to unveil a compound that fulfils the criteria as a promising anticancer agent. This research is of the utmost importance, as it provides a fundamental framework for the identification of alternative therapies for breast cancer and contributes implicitly to the development of new drugs.
Conclusions
This study discovered that
P. glabra
crude leaf extracts have the potential to inhibit the MCF-7 cell line by inducing cell death.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><doi>10.1186/s43088-024-00490-0</doi><tpages>18</tpages><orcidid>https://orcid.org/0000-0001-7074-1144</orcidid><orcidid>https://orcid.org/0000-0002-0710-2603</orcidid><orcidid>https://orcid.org/0000-0002-5613-4919</orcidid><orcidid>https://orcid.org/0000-0001-9455-5768</orcidid><orcidid>https://orcid.org/0000-0002-0771-2559</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2314-8543 |
| ispartof | Beni-Suef University journal of basic and applied sciences, 2024-12, Vol.13 (1), p.33-18, Article 33 |
| issn | 2314-8543 2314-8535 2314-8543 |
| language | eng |
| recordid | cdi_doaj_primary_oai_doaj_org_article_3dfb741a12fe46aa92a34d3b7b770953 |
| source | Publicly Available Content Database; Springer Nature - SpringerLink Journals - Fully Open Access |
| subjects | Anemia Anticancer properties Antineoplastic drugs Bioactive compounds Biocompatibility Biological activity Breast cancer Cancer therapies Cell culture Cell death Cell morphology Cytotoxicity Drug development Drugs Ethanol Flavonoids Geographical distribution Hepatitis Herbal medicine Leaves Liquid chromatography–mass spectrometry (LC–MS) MCF-7 cell line Medicine Medicine & Public Health MTT assay Plant extracts Prismatomeris glabra Toxicity Triterpenoids |
| title | The cytotoxicity effect and identification of bioactive compounds of Prismatomeris glabra crude leaf extracts against breast cancer cells |
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