A Specific and Sensitive Aptamer-Based Digital PCR Chip for Salmonella typhimurium Detection

Food poisoning and infectious diseases caused by Salmonella typhimurium (S. typhimurium) are serious public health concerns for human health and food safety. The diversity and complexity of food matrices pose great challenges for rapid and ultra-sensitive detection of S. typhimurium in food samples....

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Bibliographic Details
Published in:Biosensors (Basel) 2022-06, Vol.12 (7), p.458
Main Authors: Suo, Yuanjie, Yin, Weihong, Zhu, Qiangyuan, Wu, Wenshuai, Cao, Wenjian, Mu, Ying
Format: Article
Language:English
Subjects:
aptamer
Aptamers
Bacteria
Biosensors
digital PCR chip
Efficiency
Food contamination
Food contamination & poisoning
Food poisoning
Food safety
Foodborne diseases
Foodborne pathogens
Infectious diseases
Mass spectrometry
Meat
Methods
Onsite
Pathogens
Priming
Public health
S. typhimurium
Salmonella
Scientific imaging
selective enrichment
sensitive detection
Silicon wafers
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Summary:Food poisoning and infectious diseases caused by Salmonella typhimurium (S. typhimurium) are serious public health concerns for human health and food safety. The diversity and complexity of food matrices pose great challenges for rapid and ultra-sensitive detection of S. typhimurium in food samples. A method capable of identification, detection, and quantification of S. typhimurium is essential for addressing these issues. In this study, aptamer-coated magnetic beads (Apt-MBs) are employed as capture bio-probes to specifically and selectively concentrate S. typhimurium in food samples. A self-priming chip-based digital PCR was then presented as another biosensor for on-site detection and quantification of S. typhimurium cells. The chip we developed was robust and did not require any external power for sample loading. The combination of Apt-MBs with an on-chip digital detection realized the integration into lab-on-a-chip-based biosensors for on-site monitoring of foodborne pathogens. It was possible to capture and detect S. typhimurium cells as low as 90 CFU/reaction with a capture efficiency of 94.5%. Additionally, the whole process only took about 2 h. This unique platform could also be used to monitor other target bacteria with high specificity and sensitivity by utilizing different aptamers. Furthermore, the platform has potential applications in point-of-care testing in the future.
ISSN:2079-6374
2079-6374
DOI:10.3390/bios12070458