Expression Study of Banana Pathogenic Resistance Genes

Banana is one of the world's most important trade commodities. However, infection of banana pathogenic fungi (Fusarium oxysporum race 4) is one of the major causes of decreasing production in Indonesia. Genetic engineering has become an alternative way to control this problem by isolating genes...

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Bibliographic Details
Published in:Hayati : journal of biosciences 2016-10, Vol.23 (4), p.196-199
Main Authors: Dwivany, Fenny M., Esyanti, Rizkita Rahmi, Pratiwi, Aksarani 'Sa, Zaskia, Herafi
Format: Article
Language:English
Subjects:
Amino acids
Apoptosis
apoptosis inhibitor 5 (API5)
Arabidopsis thaliana
Bacteria
Bananas
Chitinase
chitinase (ChiI1)
Commodities
Disease
Enzymes
Flowers & plants
Fungi
Fusarium oxysporum
Gene amplification
Gene expression
Genes
Genetic engineering
Genetic improvement
Genomics
Localization
Methods
Musa acuminata
pathogen resistance
Proteins
Studies
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Summary:Banana is one of the world's most important trade commodities. However, infection of banana pathogenic fungi (Fusarium oxysporum race 4) is one of the major causes of decreasing production in Indonesia. Genetic engineering has become an alternative way to control this problem by isolating genes that involved in plant defense mechanism against pathogens. Two of the important genes are API5 and ChiI1, each gene encodes apoptosis inhibitory protein and chitinase enzymes. The purpose of this study was to study the expression of API5 and ChiI1 genes as candidate pathogenic resistance genes. The amplified fragments were then cloned, sequenced, and confirmed with in silico studies. Based on sequence analysis, it is showed that partial API5 gene has putative transactivation domain and ChiI1 has 9 chitinase family GH19 protein motifs. Data obtained from this study will contribute in banana genetic improvement.
ISSN:1978-3019
2086-4094
DOI:10.1016/j.hjb.2016.06.007