Simultaneous determination of pioglitazone and candesartan in human plasma by LC-MS/MS and its application to a human pharmacokinetic study

A simple and rapid liquid chromatography-tandem mass spectrometric(LC-MS/MS) assay method has been developed and fully validated for simultaneous quantification of pioglitazone and candesartan in human plasma.Irbesartan was used as an internal standard.The analytes were extracted from human plasma s...

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Bibliographic Details
Published in:Journal of pharmaceutical analysis 2012-06, Vol.2 (3), p.167-173
Main Authors: Kumari Karra, Vijaya, Rao Pilli, Nageswara, Kumar Inamadugu, Jaswanth, Seshagiri Rao, J.V.L.N.
Format: Article
Language:English
Subjects:
Candesartan
Human plasma
LC-MS/MS
Pharmacokinetics
Pioglitazone
Solid-phase extraction
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Summary:A simple and rapid liquid chromatography-tandem mass spectrometric(LC-MS/MS) assay method has been developed and fully validated for simultaneous quantification of pioglitazone and candesartan in human plasma.Irbesartan was used as an internal standard.The analytes were extracted from human plasma samples by solid-phase extraction technique using a Strata-X 33 mm polymeric sorbent.The reconstituted samples were chromatographed on a C18 column by using a 80:20(v/v) mixture of acetonitrile and 0.1% formic aci...
ISSN:2095-1779
2214-0883
DOI:10.1016/j.jpha.2012.01.002