Potential of endophytic fungi as a pathogenic biocontrol agent and growth promoters in corn seedlings

• Published in: Egyptian journal of biological pest control 2023-12, Vol.33 (1), p.83-7, Article 83
• Main Authors: Fauriah, Ria, Djaya, Ernawati, Djaenuddin, Nurasiah, Muis, Amran, Nonci, Nurnina
• Format: Article
• Language: English
• Subjects: Aspergillus; Biological control; Biomedical and Life Sciences; Bipolaris maydis; Blight; Blotter test; Chitinase; Control methods; Corn; Curvularia; Dual culture; Effectiveness; Endophytes; Fungi; Fusarium; Fusarium sp; Leaf blight; Leafspot; Leaves; Life Sciences; Pathogens; Pesticides; Phosphates; Seedlings; Trichoderma; Trichoderma sp; Vegetables; Zoology
• ISSN: 2536-9342; 1110-1768; 2536-9342
• DOI: 10.1186/s41938-023-00728-6

Background Use of endophytic fungi, as pathogen control of Bipolaris maydis and Curvularia sp., is an alternative method of control without the use of synthetic pesticides that are more environmentally friendly. This study aimed to determine the potential of endophytic fungi in controlling the growth of pathogens B. maydis and Curvularia sp. in vitro and in spurring the growth of corn plants. It was consisted of three types of testing three endophytic fungal species ( Aspergillus _1, Fusarium _2, and Trichoderma _11), namely (1) testing the antagonistic activity of endophytic fungal against pathogens by double culture method, (2) physiological characterization of endophytic fungal as phosphate solvents and chitinase producers, and (3) testing of corn seed vigor with the blotter test method. Results The results of testing endophytic fungal isolates against B. maydis pathogens showed that the three isolates were able to suppress the development of B. maydis , whereas the Trichoderma _11 isolate showed higher suppression results than others. The isolate that showed the best ability to dissolve phosphates is Fusarium_2 with a dissolving index of 1.9 and their effectiveness up to 91.5%. Meanwhile, Trichoderma _11 was able to produce the highest chitinase activity index of 1.9 with an effectiveness of 90.6%. The best corn root lengths and plant height were shown on Fusarium _2 treatment. Similar outcomes were observed when Curvularia sp. was tested. The whole isolates were able to suppress the growth of the pathogen by 16.43–40.44% on the 4th day after incubation. Trichoderma sp. isolate was 72.50% more effective at suppressing than the other two isolates. On day 11, the isolate of Aspergillus sp. was suppressed by 62.50%, while Fusarium sp. showed the lowest suppression of 59.17%. Conclusions Trichoderma_11 isolate was potentially the best biocontrol agent against maydis leaf blight and Curvularia leaf spot in vitro. Meanwhile, the Fusarium _2 isolate had promoted the growth of the corn seedlings.