New Pyrroline Isolated from Antarctic Krill-Derived Actinomycetes Nocardiopsis sp. LX-1 Combining with Molecular Networking

• Published in: Marine drugs 2023-02, Vol.21 (2), p.127
• Main Authors: Shi, Ting, Li, Yan-Jing, Wang, Ze-Min, Wang, Yi-Fei, Wang, Bo, Shi, Da-Yong
• Format: Article
• Language: English
• Subjects: Actinobacteria; Actinomyces; Actinomycetes; Agar diffusion test; Animals; Antarctic krill; Antarctic Regions; Antibacterial activity; Antibiotics; Antifungal activity; Antifungal Agents; antimicrobial activity; Antimicrobial agents; Bioactive compounds; Ciprofloxacin; Dilution; Ecosystem; Ecosystems; Euphausiacea - chemistry; Flavonoids; Food chains; Fungicides; Krill; Marine crustaceans; Marine ecosystems; Metabolism; Metabolites; Microorganisms; molecular networking; Molecular weight; Natural products; Networking; Nocardiopsis; Nocardiopsis sp. LX-1; nocarpyrroline A; Polar environments; Prochloraz; Pyrroles; pyrroline; Secondary metabolites
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md21020127

Antarctic krill ( ) of the family comprise one of the largest biomasses in the world and play a key role in the Antarctic marine ecosystem. However, the study of -derived microbes and their secondary metabolites has been limited. Chemical investigation of the secondary metabolites of the actinomycetes sp. LX-1 (in the family of ), isolated from , combined with molecular networking, led to the identification of 16 compounds - (purple nodes in the molecular network) and the isolation of one new pyrroline, nocarpyrroline A ( ), along with 11 known compounds - . The structure of the new compound was elucidated by extensive spectroscopic investigation. Compound exhibited broad-spectrum antibacterial activities against , , , and antifungal activity against in a conventional broth dilution assay. The positive control was ciprofloxacin with the MIC values of