Fast Adaptive Optics in optically sectioned fluorescence microscopes for functional neuroimaging

We demonstrate how a novel approach for closed-loop Adaptive Optics (AO) specifically adapted to microscopy enables straightforward integration in Light-Sheet and Multiphoton microscopes, as well as fast aberration correction. We present corresponding experimental setups as well as first demonstrati...

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Bibliographic Details
Published in:EPJ Web of conferences 2023, Vol.287, p.3005
Main Authors: Harms, Fabrice, Mercier, Mathias, Gauillaume-Manca, Alice, Veilly, Cynthia, Levecq, Xavier, Bourdieu, Laurent, Fragola, Alexandra
Format: Article
Language:English
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Summary:We demonstrate how a novel approach for closed-loop Adaptive Optics (AO) specifically adapted to microscopy enables straightforward integration in Light-Sheet and Multiphoton microscopes, as well as fast aberration correction. We present corresponding experimental setups as well as first demonstrations of the benefits of the correction of sample-induced aberrations in zebrafish and mouse brain tissue, with the ultimate goal to enable high-speed, high-sensitivity functional imaging at large depths.
ISSN:2100-014X
2100-014X
DOI:10.1051/epjconf/202328703005