The GPI-Anchored Protein Thy-1/CD90 Promotes Wound Healing upon Injury to the Skin by Enhancing Skin Perfusion

Wound healing is a highly regulated multi-step process that involves a plethora of signals. Blood perfusion is crucial in wound healing and abnormalities in the formation of new blood vessels define the outcome of the wound healing process. Thy-1 has been implicated in angiogenesis and silencing of...

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Bibliographic Details
Published in:International journal of molecular sciences 2022-10, Vol.23 (20), p.12539
Main Authors: Pérez, Leonardo A., León, José, López, Juan, Rojas, Daniela, Reyes, Montserrat, Contreras, Pamela, Quest, Andrew F. G., Escudero, Carlos, Leyton, Lisette
Format: Article
Language:English
Subjects:
Abnormalities
Angiogenesis
Biopsy
Blood circulation
blood perfusion
Blood vessels
CD90 antigen
Dermis
Epidermis
Extracellular matrix
Fourier analysis
Image acquisition
Image contrast
Lasers
Lymphocytes
Metabolism
Morphology
Neutrophils
Perfusion
Rodents
Signal processing
Skin
Skin injuries
Thy-1 (CD90)
Wound healing
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Summary:Wound healing is a highly regulated multi-step process that involves a plethora of signals. Blood perfusion is crucial in wound healing and abnormalities in the formation of new blood vessels define the outcome of the wound healing process. Thy-1 has been implicated in angiogenesis and silencing of the Thy-1 gene retards the wound healing process. However, the role of Thy-1 in blood perfusion during wound closure remains unclear. We proposed that Thy-1 regulates vascular perfusion, affecting the healing rate in mouse skin. We analyzed the time of recovery, blood perfusion using Laser Speckle Contrast Imaging, and tissue morphology from images acquired with a Nanozoomer tissue scanner. The latter was assessed in a tissue sample taken with a biopsy punch on several days during the wound healing process. Results obtained with the Thy-1 knockout (Thy-1−/−) mice were compared with control mice. Thy-1−/− mice showed at day seven, a delayed re-epithelialization, increased micro- to macro-circulation ratio, and lower blood perfusion in the wound area. In addition, skin morphology displayed a flatter epidermis, fewer ridges, and almost no stratum granulosum or corneum, while the dermis was thicker, showing more fibroblasts and fewer lymphocytes. Our results suggest a critical role for Thy-1 in wound healing, particularly in vascular dynamics.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms232012539