An antibacterial compound pyrimidomycin produced by Streptomyces sp. PSAA01 isolated from soil of Eastern Himalayan foothill

• Published in: Scientific reports 2022-06, Vol.12 (1), p.10176-10176, Article 10176
• Main Authors: Das, Prasenjit, Kundu, Shampa, Maiti, Pulak Kumar, Mandal, Saurodeep, Sahoo, Prithidipa, Mandal, Sukhendu
• Format: Article
• Language: English
• Subjects: 631/326/22; 631/326/22/1290; 631/326/22/1434; 631/326/2522; Ampicillin; Antibiotics; Bacteria; Biofilms; Cell culture; Cell lines; Cell walls; Chloramphenicol; Culture media; Cytotoxicity; Drug resistance; Gram-positive bacteria; Humanities and Social Sciences; Minimum inhibitory concentration; Molecular weight; multidisciplinary; Multidrug resistance; Science; Science (multidisciplinary); Staphylococcus aureus; Streptomyces; Vancomycin
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-022-14549-4

Selective isolation of soil Actinobacteria was undertaken to isolate a new class of antibiotics and bioactive molecules. A Streptomyces sp . PSAA01 (= MTCC 13,157), isolated from soil of Eastern Himalaya foothill was cultivated on a large scale for the production of the antimicrobial SM02. It has been found that the maximum amount of SM02 produced while PSAA01 was grown in ISP-2 medium (pH 7.0) for 7 days at 30 °C in shaking (180 rpm) condition. A significant zone of inhibition against Staphylococcus aureus MTCC 96 has been found with the crude cell-free culture media (50 µL) of 7 days grown PSAA01. After the purification and chemical structural characterization, we found that SM02 is a new antimicrobial having 746 dalton molecular weight. The compound SM02 contains pyrimidine moiety in it and is produced by a species of Streptomyces and thus we have named this antibiotic pyrimidomycin . The antimicrobial spectrum of pyrimidomycin has been found to be restricted in Gram-positive organisms with a MIC of 12 µg/mL. SM02 was found active against Mycobacterium sp. and also multi-drug resistant Gram-positive bacteria with similar potency and found to disrupt the bacterial cell wall. Pyrimidomycin also showed significant impairment in the biofilm formation by S. aureus . Furthermore, pyrimidomycin showed synergy with the most used antibiotic like ampicillin, vancomycin and chloramphenicol. Pyrimidomycin did not have cytotoxicity towards human cell lines indicating its limited activity within bacteria.