Deoxynivalenol and Zearalenone: Different Mycotoxins with Different Toxic Effects in the Sertoli Cells of Equus asinus

(1) Background: Deoxynivalenol (DON) and zearalenone (ZEA) are type B trichothecene mycotoxins that exert serious toxic effects on the reproduction of domestic animals. However, there is little information about the toxicity of mycotoxins on testis development in Equus asinus. This study investigate...

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Bibliographic Details
Published in:Cells (Basel, Switzerland) Switzerland), 2021-07, Vol.10 (8), p.1898
Main Authors: Song, Jun-Lin, Zhang, Guo-Liang
Format: Article
Language:English
Subjects:
Animals
Antibodies
Apoptosis
Deoxynivalenol
Domestic animals
DON
Equus asinus
Estrogens
Feeds
Flow cytometry
Gene expression
Immunofluorescence
Microscopy
Mycotoxins
Polymerase chain reaction
Proteins
Pyroptosis
Reproductive system
RNA-seq
Sertoli cells
Spermatogenesis
Testes
Toxicity
ZEA
Zearalenone
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Summary:(1) Background: Deoxynivalenol (DON) and zearalenone (ZEA) are type B trichothecene mycotoxins that exert serious toxic effects on the reproduction of domestic animals. However, there is little information about the toxicity of mycotoxins on testis development in Equus asinus. This study investigated the biological effects of DON and ZEA exposure on Sertoli cells (SCs) of Equus asinus; (2) Methods: We administered 10 μM and 30 μM DON and ZEA to cells cultured in vitro; (3) Results: The results showed that 10 μM DON exposure remarkably changed pyroptosis-associated genes and that 30 μM ZEA exposure changed inflammation-associated genes in SCs. The mRNA expression of cancer-promoting genes was remarkably upregulated in the cells exposed to DON or 30 μM ZEA; in particular, DON and ZEA remarkably disturbed the expression of androgen and oestrogen secretion-related genes. Furthermore, quantitative RT-PCR, Western blot, and immunofluorescence analyses verified the different expression patterns of related genes in DON- and ZEA-exposed SCs; (4) Conclusions: Collectively, these results illustrated the impact of exposure to different toxins and concrete toxicity on the mRNA expression of SCs from Equus asinus in vitro.
ISSN:2073-4409
2073-4409
DOI:10.3390/cells10081898