Antibacterial activity of iron oxide nanoparticles synthesized by co-precipitation technology against Bacillus cereus and Klebsiella pneumoniae

• Published in: Polish journal of chemical technology 2017-12, Vol.19 (4), p.110-115
• Main Authors: Ansari, Shakeel Ahmed, Oves, Mohammad, Satar, Rukhsana, Khan, Anish, Ahmad, Syed Ismail, Jafri, Mohammad Alam, Zaidi, Syed Kashif, Alqahtani, Mohammad Husein
• Format: Article
• Language: English
• Subjects: antibacterial activity; co-precipitation; iron oxide nanoparticles; Klebsiella pneumoniae; physico-chemical characterization
• ISSN: 1899-4741; 1899-4741
• DOI: 10.1515/pjct-2017-0076

The present study investigates the synthesis and characterization of iron oxide nanoparticles (Fe -NPs) for their antibacterial potential against and by modified disc diffusion and broth agar dilution methods. DLS and XRD results revealed the average size of synthesized Fe -NPs as 24 nm while XPS measurement exhibited the spin-orbit peak of Fe 2p binding energy at 511 eV. Fe -NPs inhibited the growth of and in both liquid and soild agar media, and displayed 26 mm and 22 mm zone of inhibitions, respectively. MIC of Fe -NPs was found to be 5 μg/mL against these strains. However, MBC for these strains was observed at 40 μg/mL concentration of Fe -NPs for exhibiting 40–50% loss in viable bacterial cells and 80 μg/mL concentration of Fe -NPs acted as bactericidal for causing 90–99% loss in viability. Hence, these nanoparticles can be explored for their additional antimicrobial and biomedical applications.