Simultaneous transfer of cholesterol, triglycerides, and phospholipids to high-density lipoprotein in aging subjects with or without coronary artery disease

• Published in: Clinics (São Paulo, Brazil) Brazil), 2011-01, Vol.66 (9), p.1543-1548
• Main Authors: Azevedo, Carolina H.M., Wajngarten, Maurício, Prete, Ana C. Lo, Diament, Jayme, Maranhão, Raul C.
• Format: Article
• Language: English
• Subjects: Adult; Aged; Aged, 80 and over; Aging; Aging - blood; Aryldialkylphosphatase - blood; Cholesterol Esters - blood; Cholesterol, HDL - blood; cholesteryl ester transfer protein (CETP); Clinical Science; Coronary Artery Disease - blood; Emulsions; Epidemiologic Methods; Female; Humans; lipoproteins; Male; MEDICINE, GENERAL & INTERNAL; Middle Aged; Nanoparticles; Particle Size; Phospholipids - blood; transfer proteins; Triglycerides - blood
• ISSN: 1807-5932; 1980-5322; 1980-5322
• DOI: 10.1590/S1807-59322011000900006

To verify whether the capacity of high-density lipoprotein (HDL) to simultaneously receive non-esterified cholesterol, triglycerides, cholesteryl esters, and phospholipids changes with aging and the presence of coronary artery disease. Cross-sectional study with biochemical analyses. Eleven elderly patients with coronary artery disease (74±5 years) were compared with the following groups of non-coronary artery disease subjects (referred to as “healthy”): 25 young (25±5 years), 25 middle-aged (42±6 years), and 25 elderly subjects (75±8 years). Plasma samples were incubated with a nanoemulsion labeled with radioactive lipids; the transfer of the lipids from the nanoemulsion to the HDL was measured in chemically precipitated HDL. HDL size and paraoxonase-1 activity were also determined. The transfer of cholesteryl esters and phospholipids to high-density lipoprotein was significantly greater (p