Simultaneous transfer of cholesterol, triglycerides, and phospholipids to high-density lipoprotein in aging subjects with or without coronary artery disease

To verify whether the capacity of high-density lipoprotein (HDL) to simultaneously receive non-esterified cholesterol, triglycerides, cholesteryl esters, and phospholipids changes with aging and the presence of coronary artery disease. Cross-sectional study with biochemical analyses. Eleven elderly...

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Published in:Clinics (São Paulo, Brazil) Brazil), 2011-01, Vol.66 (9), p.1543-1548
Main Authors: Azevedo, Carolina H.M., Wajngarten, Maurício, Prete, Ana C. Lo, Diament, Jayme, Maranhão, Raul C.
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Aging
Aging - blood
Aryldialkylphosphatase - blood
Cholesterol Esters - blood
Cholesterol, HDL - blood
cholesteryl ester transfer protein (CETP)
Clinical Science
Coronary Artery Disease - blood
Emulsions
Epidemiologic Methods
Female
Humans
lipoproteins
Male
MEDICINE, GENERAL & INTERNAL
Middle Aged
Nanoparticles
Particle Size
Phospholipids - blood
transfer proteins
Triglycerides - blood
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container_issue 9
container_start_page 1543
container_title Clinics (São Paulo, Brazil)
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creator Azevedo, Carolina H.M.
Wajngarten, Maurício
Prete, Ana C. Lo
Diament, Jayme
Maranhão, Raul C.
description To verify whether the capacity of high-density lipoprotein (HDL) to simultaneously receive non-esterified cholesterol, triglycerides, cholesteryl esters, and phospholipids changes with aging and the presence of coronary artery disease. Cross-sectional study with biochemical analyses. Eleven elderly patients with coronary artery disease (74±5 years) were compared with the following groups of non-coronary artery disease subjects (referred to as “healthy”): 25 young (25±5 years), 25 middle-aged (42±6 years), and 25 elderly subjects (75±8 years). Plasma samples were incubated with a nanoemulsion labeled with radioactive lipids; the transfer of the lipids from the nanoemulsion to the HDL was measured in chemically precipitated HDL. HDL size and paraoxonase-1 activity were also determined. The transfer of cholesteryl esters and phospholipids to high-density lipoprotein was significantly greater (p
doi_str_mv 10.1590/S1807-59322011000900006
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The transfer of cholesteryl esters and phospholipids to high-density lipoprotein was significantly greater (p&lt;0.001) in healthy elderly subjects than in the middle-aged and younger subjects. Non-esterified cholesterol and triglyceride transfer was not different among these three groups. The HDL size was significantly greater (p&lt;0.001) in healthy elderly subjects than in the middle-aged and younger subjects. The paraoxonase-1 activity was similar among the groups. Compared with healthy elderly subjects, coronary artery disease elderly subjects had significantly less (p&lt;0.05) transfer of non-esterified cholesterol, triglycerides, and cholesteryl esters to the HDL and a significantly smaller (p&lt;0.05) HDL size. 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Compared with healthy elderly subjects, coronary artery disease elderly subjects had significantly less (p&lt;0.05) transfer of non-esterified cholesterol, triglycerides, and cholesteryl esters to the HDL and a significantly smaller (p&lt;0.05) HDL size. Because lipid transfer is enhanced in healthy elderly subjects but not in those with coronary artery disease, increasing lipid transfer to HDL may be a protective mechanism against the disease.</abstract><cop>Brazil</cop><pub>Elsevier España, S.L.U</pub><pmid>22179156</pmid><doi>10.1590/S1807-59322011000900006</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record>
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ispartof Clinics (São Paulo, Brazil), 2011-01, Vol.66 (9), p.1543-1548
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source SciELO Brazil; ScienceDirect Journals; PubMed Central
subjects Adult
Aged
Aged, 80 and over
Aging
Aging - blood
Aryldialkylphosphatase - blood
Cholesterol Esters - blood
Cholesterol, HDL - blood
cholesteryl ester transfer protein (CETP)
Clinical Science
Coronary Artery Disease - blood
Emulsions
Epidemiologic Methods
Female
Humans
lipoproteins
Male
MEDICINE, GENERAL & INTERNAL
Middle Aged
Nanoparticles
Particle Size
Phospholipids - blood
transfer proteins
Triglycerides - blood
title Simultaneous transfer of cholesterol, triglycerides, and phospholipids to high-density lipoprotein in aging subjects with or without coronary artery disease
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