Connexin 26 is down-regulated by KDM5B in the progression of bladder cancer

Connexin 26 (Cx26) expression is down-regulated and KDM5B (H3K4 demethylase) is up-regulated in the progression of bladder cancer, suggesting that Cx26 expression may be down-regulated by KDM5B in bladder cancer. To test the hypothesis, the HT1376 and T24 human bladder carcinoma cells were transfect...

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Bibliographic Details
Published in:International journal of molecular sciences 2013-04, Vol.14 (4), p.7866-7879
Main Authors: Li, Xin, Su, Yongping, Pan, Jinhong, Zhou, Zhansong, Song, Bo, Xiong, Enqing, Chen, Zhiwen
Format: Article
Language:English
Subjects:
Bladder cancer
Cell Line, Tumor
Connexin 26
Connexins - biosynthesis
Connexins - genetics
Down-Regulation
Gene Expression Regulation, Neoplastic
HT1376 human bladder cancer cells
Humans
Immunohistochemistry
Jumonji Domain-Containing Histone Demethylases - genetics
Jumonji Domain-Containing Histone Demethylases - metabolism
KDM5B
Neoplasm Proteins - genetics
Neoplasm Proteins - metabolism
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
real-time qPCR
Repressor Proteins - genetics
Repressor Proteins - metabolism
spearman’s rank correlation
T24 human bladder cancer cells
Urinary Bladder Neoplasms - genetics
Urinary Bladder Neoplasms - metabolism
Urinary Bladder Neoplasms - pathology
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Summary:Connexin 26 (Cx26) expression is down-regulated and KDM5B (H3K4 demethylase) is up-regulated in the progression of bladder cancer, suggesting that Cx26 expression may be down-regulated by KDM5B in bladder cancer. To test the hypothesis, the HT1376 and T24 human bladder carcinoma cells were transfected with the plasmids pcDNA3.1-KDM5B, and caused the down-regulation of Cx26 expression. In contrast, the HT1376 and T24 cells transfected with the plasmids pTZU6+1-shRNA-KDM5B1 and pTZU6+1-shRNA-KDM5B2 caused the up-regulation of Cx26 expression. Immunohistochemistry and Spearman's rank correlation analysis showed that the immunohistochemical expression of KDM5B and Cx26 was inversely related in bladder carcinoma tissues but no relationship in benign tissues. Taken together, these results indicate that KDM5B represses Cx26 expression in the bladder cancer development. Thus, a negative value to Cx26 immunohistochemical expression and a positive value to KDM5B immunohistochemical expression could be an ancillary diagnosis of primary bladder malignancy.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms14047866