In Vitro Biological and GC-MS Analysis of Whole Plant Calotropis procera

Calotropis procera is a medicinal, traditional, and therapeutic plant in Pakistan. In this research study, the biological activities, for instance, anticancer, antileishmanial, antibacterial, antifungal, anti-inflammatory, and brine shrimp lethality assay and GC-MS are studied. MTT assay, antileishm...

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Published in:Journal of chemistry 2024-05, Vol.2024, p.1-23
Main Authors: Shah, Qahir, Achakzai, Jahangir Khan, Panezai, Muhammad Anwar, Akhtar, Basira, Kakar, Abdul Manan, Akbar, Ali, Kakar, Shahabuddin, Shahwani, Nisar Ahmed, Khan, Javed, Khan, Nazima Yousaf, Khan, Ghulam Mustafa, Baloch, Nizam, Rahim, Bakht Zareen, Hassan, Noor
Format: Article
Language:English
Subjects:
Alcohol industry
Analysis
Anticancer properties
Antifungal agents
Antiinfectives and antibacterials
Asthma
Bioassays
Brines
Chemiluminescence
Dilution
Flowers & plants
Fungicides
Gas chromatography
Health aspects
Herbal medicine
Hexanes
Leaves
Lethality
Mass spectrometry
Methanol
Pentamidine isethionate
Quadrupoles
Seeds
Shrimps
Skin diseases
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Summary:Calotropis procera is a medicinal, traditional, and therapeutic plant in Pakistan. In this research study, the biological activities, for instance, anticancer, antileishmanial, antibacterial, antifungal, anti-inflammatory, and brine shrimp lethality assay and GC-MS are studied. MTT assay, antileishmanial bioassay, microplate Alamar Blue assay, agar tube dilution method, oxidative burst assay using chemiluminescence technique, B-hatching techniques, and triple quadrupole acquisition method MS parameters were the methods used for anticancer, antileishmanial, antibacterial, antifungal, anti-inflammatory, brine shrimp lethality assay, and gas chromatography-mass spectrometry. Whole plant methanol extract of Calotropis procera (WMECP) inhibited 69.1% of the growth of HeLa cell line with an IC50 value of 3.1 ± 0.4 and whole plant n-hexane fraction of Calotropis procera (WHFCP) and whole plant and aqueous fraction of Calotropis procera (WAFCP) inhibited the growth by 70.2% and 65.2% with IC50 values of 5.0 ± 0.3 and 17.1 ± 1.0. Whole plant methanol extract of Calotropis procera (WMECP) inhibited 70.1% of the growth of the PC3 cell line with an IC50 value of 5.1 ± 0.3 and whole plant n-hexane fraction of Calotropis procera (WHFCP) and whole plant aqueous fraction of Calotropis procera (WAFCP) inhibited 61.6% and 59.7% with IC50 values of 3.7 ± 0.5 and 16.4 ± 1.0. None of the extract and fractions of Calotropis procera showed anticancer activities against the 3T3 cell line. None of the extract and fractions of Calotropis procera showed antileishmanial, antibacterial, antifungal, and anti-inflammatory activities. Whole plant methanol extract of Calotropis procera (WMECP) exhibited lethality at the highest concentration while other fractions did not exhibit lethality. GC-MS studies revealed that the whole plant methanol extract of Calotropis procera (WMECP) consists of 11 compounds, whole plant n-hexane fraction of Calotropis procera (WHFCP) consists of 9 compounds, and whole plant aqueous fraction of Calotropis procera (WAFCP) consists of 7 compounds.
ISSN:2090-9063
2090-9071
DOI:10.1155/2024/2012983