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Regulation of dndB Gene Expression in Streptomyces lividans
DNA sulfur modification is a unique modification occurring in the sugar-phosphate backbone of DNA, with a nonbridging oxygen atom substituted with sulfur in a sequence-specific and p stereo-specific manner. Bioinformatics, RNA-seq, and transcriptional analyses have shown that DNA sulfur modification...
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| Published in: | Frontiers in microbiology 2018-10, Vol.9, p.2387-2387 |
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| Main Authors: | , , , , |
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| Language: | English |
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| container_title | Frontiers in microbiology |
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| creator | Dai, Daofeng Pu, Tianning Liang, Jingdan Wang, Zhijun Tang, Aifa |
| description | DNA sulfur modification is a unique modification occurring in the sugar-phosphate backbone of DNA, with a nonbridging oxygen atom substituted with sulfur in a sequence-specific and
p stereo-specific manner. Bioinformatics, RNA-seq, and
transcriptional analyses have shown that DNA sulfur modification may be involved in epigenetic regulation. However, the
evidence supporting this assertion is not convincing. Here, we aimed to characterize two sulfur-modified sites near the
promoter region in
. Single mutation of either site had no effect on
transcription, whereas double mutation of both sites significantly elevated
expression. These findings suggested that DNA sulfur modification affected gene expression, and the role of DNA sulfur modification in epigenetic regulation depended on the number of sulfur-modified sites. We also identified an inverted repeat, the R repeat sequence, and showed that this sequence participated in the positive regulation of
gene expression. |
| doi_str_mv | 10.3389/fmicb.2018.02387 |
| format | article |
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p stereo-specific manner. Bioinformatics, RNA-seq, and
transcriptional analyses have shown that DNA sulfur modification may be involved in epigenetic regulation. However, the
evidence supporting this assertion is not convincing. Here, we aimed to characterize two sulfur-modified sites near the
promoter region in
. Single mutation of either site had no effect on
transcription, whereas double mutation of both sites significantly elevated
expression. These findings suggested that DNA sulfur modification affected gene expression, and the role of DNA sulfur modification in epigenetic regulation depended on the number of sulfur-modified sites. We also identified an inverted repeat, the R repeat sequence, and showed that this sequence participated in the positive regulation of
gene expression.</description><identifier>ISSN: 1664-302X</identifier><identifier>EISSN: 1664-302X</identifier><identifier>DOI: 10.3389/fmicb.2018.02387</identifier><identifier>PMID: 30349518</identifier><language>eng</language><publisher>Switzerland: Frontiers Media S.A</publisher><subject>DNA sulfur modification ; dndB gene ; epigenetic regulation ; Microbiology ; Streptomyces lividans ; transcriptional regulation</subject><ispartof>Frontiers in microbiology, 2018-10, Vol.9, p.2387-2387</ispartof><rights>Copyright © 2018 Dai, Pu, Liang, Wang and Tang. 2018 Dai, Pu, Liang, Wang and Tang</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c462t-be73558c0ee38833806c489651a0a154f47ba34ffcc0f1a1fdb3a15ac65a9f893</citedby><cites>FETCH-LOGICAL-c462t-be73558c0ee38833806c489651a0a154f47ba34ffcc0f1a1fdb3a15ac65a9f893</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6186775/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6186775/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30349518$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dai, Daofeng</creatorcontrib><creatorcontrib>Pu, Tianning</creatorcontrib><creatorcontrib>Liang, Jingdan</creatorcontrib><creatorcontrib>Wang, Zhijun</creatorcontrib><creatorcontrib>Tang, Aifa</creatorcontrib><title>Regulation of dndB Gene Expression in Streptomyces lividans</title><title>Frontiers in microbiology</title><addtitle>Front Microbiol</addtitle><description>DNA sulfur modification is a unique modification occurring in the sugar-phosphate backbone of DNA, with a nonbridging oxygen atom substituted with sulfur in a sequence-specific and
p stereo-specific manner. Bioinformatics, RNA-seq, and
transcriptional analyses have shown that DNA sulfur modification may be involved in epigenetic regulation. However, the
evidence supporting this assertion is not convincing. Here, we aimed to characterize two sulfur-modified sites near the
promoter region in
. Single mutation of either site had no effect on
transcription, whereas double mutation of both sites significantly elevated
expression. These findings suggested that DNA sulfur modification affected gene expression, and the role of DNA sulfur modification in epigenetic regulation depended on the number of sulfur-modified sites. We also identified an inverted repeat, the R repeat sequence, and showed that this sequence participated in the positive regulation of
gene expression.</description><subject>DNA sulfur modification</subject><subject>dndB gene</subject><subject>epigenetic regulation</subject><subject>Microbiology</subject><subject>Streptomyces lividans</subject><subject>transcriptional regulation</subject><issn>1664-302X</issn><issn>1664-302X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNpVkU1rGzEQhkVpSUKSe05lj73Y1ddqtQQKbXDTgCHQpNCbmNWOHJndlSutQ_zvI380xHPQDK9mnhl4CblidCqErr-63ttmyinTU8qFrj6QM6aUnAjK_358V5-Sy5SWNIekPL8n5FRQIeuS6TNy_RsX6w5GH4YiuKId2h_FLQ5YzF5WEVPa6n4oHsaIqzH0G4up6Pyzb2FIF-STgy7h5SGfkz8_Z483vybz-9u7m-_ziZWKj5MGK1GW2lJEoXU-nSorda1KBhRYKZ2sGhDSOWupY8Bc24isg1Ul1E7X4pzc7bltgKVZRd9D3JgA3uyEEBcG4uhth0ZkgHZWARdSsqqtK5W3IqiGOS4ryKxve9Zq3fTYWhzGCN0R9Phn8E9mEZ6NYlpVVZkBXw6AGP6tMY2m98li18GAYZ0MZ1zVVKqa5Va6b7UxpBTRva1h1GwtNDsLzdZCs7Mwj3x-f97bwH_DxCuHY5il</recordid><startdate>20181008</startdate><enddate>20181008</enddate><creator>Dai, Daofeng</creator><creator>Pu, Tianning</creator><creator>Liang, Jingdan</creator><creator>Wang, Zhijun</creator><creator>Tang, Aifa</creator><general>Frontiers Media S.A</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20181008</creationdate><title>Regulation of dndB Gene Expression in Streptomyces lividans</title><author>Dai, Daofeng ; Pu, Tianning ; Liang, Jingdan ; Wang, Zhijun ; Tang, Aifa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c462t-be73558c0ee38833806c489651a0a154f47ba34ffcc0f1a1fdb3a15ac65a9f893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>DNA sulfur modification</topic><topic>dndB gene</topic><topic>epigenetic regulation</topic><topic>Microbiology</topic><topic>Streptomyces lividans</topic><topic>transcriptional regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dai, Daofeng</creatorcontrib><creatorcontrib>Pu, Tianning</creatorcontrib><creatorcontrib>Liang, Jingdan</creatorcontrib><creatorcontrib>Wang, Zhijun</creatorcontrib><creatorcontrib>Tang, Aifa</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Frontiers in microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dai, Daofeng</au><au>Pu, Tianning</au><au>Liang, Jingdan</au><au>Wang, Zhijun</au><au>Tang, Aifa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regulation of dndB Gene Expression in Streptomyces lividans</atitle><jtitle>Frontiers in microbiology</jtitle><addtitle>Front Microbiol</addtitle><date>2018-10-08</date><risdate>2018</risdate><volume>9</volume><spage>2387</spage><epage>2387</epage><pages>2387-2387</pages><issn>1664-302X</issn><eissn>1664-302X</eissn><abstract>DNA sulfur modification is a unique modification occurring in the sugar-phosphate backbone of DNA, with a nonbridging oxygen atom substituted with sulfur in a sequence-specific and
p stereo-specific manner. Bioinformatics, RNA-seq, and
transcriptional analyses have shown that DNA sulfur modification may be involved in epigenetic regulation. However, the
evidence supporting this assertion is not convincing. Here, we aimed to characterize two sulfur-modified sites near the
promoter region in
. Single mutation of either site had no effect on
transcription, whereas double mutation of both sites significantly elevated
expression. These findings suggested that DNA sulfur modification affected gene expression, and the role of DNA sulfur modification in epigenetic regulation depended on the number of sulfur-modified sites. We also identified an inverted repeat, the R repeat sequence, and showed that this sequence participated in the positive regulation of
gene expression.</abstract><cop>Switzerland</cop><pub>Frontiers Media S.A</pub><pmid>30349518</pmid><doi>10.3389/fmicb.2018.02387</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Frontiers in microbiology, 2018-10, Vol.9, p.2387-2387 |
| issn | 1664-302X 1664-302X |
| language | eng |
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| source | PubMed Central |
| subjects | DNA sulfur modification dndB gene epigenetic regulation Microbiology Streptomyces lividans transcriptional regulation |
| title | Regulation of dndB Gene Expression in Streptomyces lividans |
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