Characterization and Full Genome Sequence of Novel KPP-5 Lytic Phage against Klebsiella pneumoniae Responsible for Recalcitrant Infection

is a hazardous opportunistic pathogen that is involved in many serious human diseases and is considered to be an important foodborne pathogen found in many food types. Multidrug resistance (MDR) strains have recently spread and increased, making bacteriophage therapy an effective alternative to mult...

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Bibliographic Details
Published in:Biomedicines 2021-03, Vol.9 (4), p.342
Main Authors: Sofy, Ahmed R, El-Dougdoug, Noha K, Refaey, Ehab E, Dawoud, Rehab A, Hmed, Ahmed A
Format: Article
Language:English
Subjects:
Antibiotics
Autographviridae
Bacteria
Bacterial infections
Bacteriophage
Biofilms
Biological control
Burst size
Drug resistance
Electron microscopy
Enzymes
Experiments
Food
Foodborne pathogens
Full genome
Genomes
Host range
Klebsiella pneumoniae
Latent period
Microscopy
Multidrug resistance
Nosocomial infections
Nucleotide sequence
Open reading frames
Opportunist infection
phage therapy
Phages
Phylogeny
Proteins
tRNA
Virulence
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Summary:is a hazardous opportunistic pathogen that is involved in many serious human diseases and is considered to be an important foodborne pathogen found in many food types. Multidrug resistance (MDR) strains have recently spread and increased, making bacteriophage therapy an effective alternative to multiple drug-resistant pathogens. As a consequence, this research was conducted to describe the genome and basic biological characteristics of a novel phage capable of lysing MDR isolated from food samples in Egypt. The host range revealed that KPP-5 phage had potent lytic activity and was able to infect all selected MDR strains from different sources. Electron microscopy images showed that KPP-5 lytic phage was a podovirus morphology. The one-step growth curve exhibited that KPP-5 phage had a relatively short latent period of 25 min, and the burst size was about 236 PFU/infected cells. In addition, KPP-5 phage showed high stability at different temperatures and pH levels. KPP-5 phage has a linear dsDNA genome with a length of 38,245 bp with a GC content of 50.8% and 40 predicted open reading frames (ORFs). Comparative genomics and phylogenetic analyses showed that KPP-5 is most closely associated with the genus in the family. No tRNA genes have been identified in the KPP-5 phage genome. In addition, phage-borne virulence genes or drug resistance genes were not present, suggesting that KPP-5 could be used safely as a phage biocontrol agent.
ISSN:2227-9059
2227-9059
DOI:10.3390/biomedicines9040342