Cuticle deposition duration in the uterus is correlated with eggshell cuticle quality in White Leghorn laying hens

The cuticle formed in the uterus is the outermost layer as the first defense line of eggshell against microbial invasions in most avian species, and analyzing its genetic regulation and influencing factors are of great importance to egg biosecurity in poultry production worldwide. The current study...

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Bibliographic Details
Published in:Scientific reports 2021-11, Vol.11 (1), p.22100-22100, Article 22100
Main Authors: Chen, Xia, He, Zhaoxiang, Li, Xingzheng, Song, Jianlou, Huang, Mingyi, Shi, Xuefeng, Li, Xianyu, Li, Junying, Xu, Guiyun, Zheng, Jiangxia
Format: Article
Language:English
Subjects:
631/208/1348
631/601/1737
Animals
Biosecurity
Chickens - genetics
Chickens - physiology
Cuticles
Egg Shell - physiology
Egg shells
Eggs
Female
Gene Expression Profiling - methods
Gene Expression Regulation - genetics
Hormones
Humanities and Social Sciences
Invasive species
multidisciplinary
Oviposition - genetics
Poultry production
Proteomes
Science
Science (multidisciplinary)
Transcriptome - genetics
Transcriptomes
Transcriptomics
Uterus
Uterus - physiology
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Summary:The cuticle formed in the uterus is the outermost layer as the first defense line of eggshell against microbial invasions in most avian species, and analyzing its genetic regulation and influencing factors are of great importance to egg biosecurity in poultry production worldwide. The current study compared the uterine transcriptome and proteome of laying hens producing eggs with good and poor cuticle quality (GC and PC, the top and tail of the cuticle quality distribution), and identified several genes involved with eggshell cuticle quality (ESCQ). Overall, transcriptomic analysis identified 53 differentially expressed genes (DEGs) between PC versus GC group hens, among which 25 were up-regulated and 28 were down-regulated. No differences were found in the uterine proteome. Several DEGs, including PTGDS , PLCG2 , ADM and PRLR related to uterine functions and reproductive hormones, were validated by qPCR analysis. Egg quality measurements between GC and PC hens showed GC hens had longer laying interval between two consecutive ovipositions (25.64 ± 1.23 vs 24.94 ± 1.12 h) and thicker eggshell thickness (352.01 ± 23.04 vs 316.20 ± 30.58 μm) ( P  
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-021-01718-0