Mare stromal endometrial cells differentially modulate inflammation depending on oestrus cycle status: an in vitro study

The modulation of inflammation is pivotal for uterine homeostasis. Here we evaluated the effect of the oestrus cycle on the expression of pro-inflammatory and anti-inflammatory markers in a cellular model of induced fibrosis. Mare endometrial stromal cells isolated from follicular or mid-luteal phas...

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Published in:Frontiers in veterinary science 2023-10, Vol.10, p.1271240-1271240
Main Authors: Wong, Yat S., Mançanares, Ana C., Navarrete, Felipe I., Poblete, Pamela M., Méndez-Pérez, Lídice, Ferreira-Dias, Graça M. L., Rodriguez-Alvarez, Lleretny, Castro, Fidel Ovidio
Format: Article
Language:English
Subjects:
anti-fibrotic miRNA
endometrium stromal cells
endometrosis
extracellular vesicles
fibrosis-related genes
pro-fibrotic miRNA
Veterinary Science
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Summary:The modulation of inflammation is pivotal for uterine homeostasis. Here we evaluated the effect of the oestrus cycle on the expression of pro-inflammatory and anti-inflammatory markers in a cellular model of induced fibrosis. Mare endometrial stromal cells isolated from follicular or mid-luteal phase were primed with 10 ng/mL of TGFβ alone or in combination with either IL1β, IL6, or TNFα (10 ng/mL each) or all together for 24 h. Control cells were not primed. Messenger and miRNA expression were analyzed using real-time quantitative PCR (RT-qPCR). Cells in the follicular phase primed with pro-inflammatory cytokines showed higher expression of collagen-related genes ( CTGF, COL1A1, COL3A1 , and TIMP1 ) and mesenchymal marker ( SLUG, VIM, CDH2 , and CDH11 ) genes; p  
ISSN:2297-1769
2297-1769
DOI:10.3389/fvets.2023.1271240