Genome-Wide Analysis Reveals Stress and Hormone Responsive Patterns of JAZ Family Genes in Camellia Sinensis

• Published in: International journal of molecular sciences 2020-03, Vol.21 (7), p.2433
• Main Authors: Shen, Jiazhi, Zou, Zhongwei, Xing, Hongqing, Duan, Yu, Zhu, Xujun, Ma, Yuanchun, Wang, Yuhua, Fang, Wanping
• Format: Article
• Language: English
• Subjects: Abiotic stress; abiotic stresses; Abscisic acid; Acetates; Amino acids; Camellia sinensis; Camellia sinensis - genetics; Correlation analysis; Cyclopentanes; Escherichia coli; expression profiles; Gene expression; Gene Expression Regulation, Plant; Genes; Genome, Plant; Genomes; Gibberellins; Hormones - genetics; JAZ; Localization; Methyl jasmonate; Multigene Family; Nuclei (cytology); Oxylipins; Phylogenetics; Phylogeny; Plant growth; Plant Proteins - genetics; Polyethylene glycol; Promoter Regions, Genetic; Proteins; Repressor Proteins - genetics; Stress, Physiological; Tea
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms21072433

JAZ (Jasmonate ZIM-domain) proteins play pervasive roles in plant development and defense reaction. However, limited information is known about the JAZ family in . In this study, 12 non-redundant genes were identified from the tea plant genome database. Phylogenetic analysis showed that the 12 JAZ proteins belong to three groups. The -elements in promoters of CsJAZ genes and CsJAZ proteins interaction networks were also analyzed. Quantitative RT-PCR analysis showed that 7 genes were preferentially expressed in roots. Furthermore, the expressions were differentially induced by cold, heat, polyethylene glycol (PEG), methyl jasmonate (MeJA), and gibberellin (GA) stimuli. The Pearson correlations analysis based on expression levels showed that the gene pairs were differentially expressed under different stresses, indicating that might exhibit synergistic effects in response to various stresses. Subcellular localization assay demonstrated that CsJAZ3, CsJAZ10, and CsJAZ11 fused proteins were localized in the cell nucleus. Additionally, the overexpression of , and in enhanced the growth of recombinant cells under abiotic stresses. In summary, this study will facilitate the understanding of the family in and provide new insights into the molecular mechanism of tea plant response to abiotic stresses and hormonal stimuli.