Improved 18S small subunit rDNA primers for problematic nematode amplification

The 18S small subunit (SSU) ribosomal DNA sequence is one of the most useful molecular loci for identification and phylogeny reconstruction of agriculturally important nematodes. Various pairs of universal primers have been developed in the past to amplify short and long nematode sequences. However,...

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Bibliographic Details
Published in:Journal of nematology 2018-01, Vol.50 (4), p.533-542
Main Authors: Carta, L. K., Li, S.
Format: Article
Language:English
Subjects:
Barcode
Life Sciences
Molecular sequence
Nematode identification
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Summary:The 18S small subunit (SSU) ribosomal DNA sequence is one of the most useful molecular loci for identification and phylogeny reconstruction of agriculturally important nematodes. Various pairs of universal primers have been developed in the past to amplify short and long nematode sequences. However, certain nematode taxa were not readily amplified and/or sequenced with the existing primer tools. Frequently, the center region of a roughly 1,000 nucleotide segment would be lost. Therefore new primers were developed based on a very large 276 taxon alignment of 124 agriculturally important nematode species, and tested on problematic nematode taxa such as , and . New primers and protocols are provided for successful generation of sequences useful in future investigations of nematode systematics.
ISSN:0022-300X
2640-396X
2640-396X
DOI:10.21307/jofnem-2018-051