In Vitro and In Vivo Wide-Spectrum Dual Antimycetomal Activity of Eight Essential Oils Coupled with Chemical Composition and Metabolomic Profiling

• Published in: Microbiology research 2024-09, Vol.15 (3), p.1280-1297
• Main Authors: Abd Algaffar, Shereen O., Satyal, Prabodh, Ashmawy, Naglaa S., Verbon, Annelies, van de Sande, Wendy W. J., Khalid, Sami A.
• Format: Article
• Language: English
• Subjects: Actinomadura madurae; Antimicrobial agents; Biocompatibility; Chemical activity; Chemical composition; Distillation; Essential oils; Fungal diseases; Fungi; Galleria mellonella; Gas chromatography; in vitro; in vivo; In vivo methods and tests; Larvae; Madurella mycetomatis; Maduromycosis; Mass spectrometry; Mass spectroscopy; Metabolism; Metabolomics; neglected tropical diseases; Oils & fats; Pathogens; Pimpinella anisum; Roots; Steam distillation; Strains (organisms); Survival; Toxicity; Toxicity testing; Tropical environment; Tropical environments
• ISSN: 2036-7481; 2036-7481
• DOI: 10.3390/microbiolres15030086

Mycetoma, a neglected infection of subcutaneous tissues, poses a significant health burden, especially in tropical regions. It is caused by fungal (eumycetoma) and bacterial (actinomycetoma) pathogens, with current treatments often providing unsatisfactory outcomes. This study aims to discover novel broad-spectrum antimicrobial agents to circumvent the lengthy and costly diagnostic procedures. Eight essential oils (EOs) from the roots and aerial parts of Geigeria alata, Lavandula angustifolia, Melaleuca alternifolia, Myristica fragrans, Pimpinella anisum, Syzigum aromaticum, and Thymus vulgaris were prepared using steam distillation. The in vitro antimycetomal activity against Madurella mycetomatis and Actinomadura madurae strains was assessed using resazurin assays. The chemical compositions of the EOs were analyzed using gas chromatography and mass spectrometry (GC–MS). Promising EOs underwent further in vivo toxicity and efficacy testing in Galleria mellonella larvae models. EOs of G. alata roots, M. fragrans, P. anisum, S. aromaticum, and T. vulgaris showed wide-spectrum dual in vitro antimycetomal activity against all tested strains, with minimum inhibitory concentrations (MICs) ranging from 0.004 to 0.125% v/v. G. alata aerial parts and L. angustifolia EOs demonstrated activity predominantly against A. madurae, while M. alternifolia EO did not inhibit any tested strains. M. fragrans and P. anisum EOs significantly enhanced the survival of M. mycetomatis-infected larvae without inducing toxicity in uninfected larvae. Notably, P. anisum EO tended to enhance the survival of A. madurae-infected larvae, ranking it as the most promising EO among those tested. The investigated EOs, particularly P. anisum, exhibited promising broad-spectrum antimycetomal activity against fungal and bacterial pathogens responsible for mycetoma. These findings highlight the potential of essential oils as a basis for developing novel antimycetomal agents, offering hope for improved treatment strategies for this neglected disease.