The first Acanthamoeba keratitis case in the Midwest region of Brazil: diagnosis, genotyping of the parasite and disease outcome

We report an Acanthamoeba keratitis case associated with the use of contact lens in a 28-year-old female from Brasília, Brazil. Samples from corneal scraping and contact lens case were used for culture establishment, PCR amplification, and partial sequencing (fragments of ~400kb) of small subunit rD...

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Bibliographic Details
Published in:Revista da Sociedade Brasileira de Medicina Tropical 2018-09, Vol.51 (5), p.716-719
Main Authors: Alves, Daniella de Sousa Mendes Moreira, Gonçalves, Gustavo Souza, Moraes, Aline Silva, Alves, Luciano Moreira, Carmo Neto, José Rodrigues do, Hecht, Mariana Machado, Nitz, Nadjar, Gurgel-Gonçalves, Rodrigo, Bernardes, Geisa, Castro, Ana Maria de, Chalita, Maria Regina, Vinaud, Marina Clare
Format: Article
Language:English
Subjects:
Acanthamoeba
Acanthamoeba - genetics
Acanthamoeba - isolation & purification
Acanthamoeba keratitis
Acanthamoeba Keratitis - diagnosis
Acanthamoeba Keratitis - etiology
Acanthamoeba Keratitis - surgery
Adult
Case reports
Contact lenses
Contact Lenses - parasitology
Cornea
Culture
Eye diseases
Female
Genotype
Genotypes
Genotyping
Humans
Keratitis
Medical diagnosis
Parasites
PCR
Scraping
TROPICAL MEDICINE
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Summary:We report an Acanthamoeba keratitis case associated with the use of contact lens in a 28-year-old female from Brasília, Brazil. Samples from corneal scraping and contact lens case were used for culture establishment, PCR amplification, and partial sequencing (fragments of ~400kb) of small subunit rDNA; both culture and PCR were positive. The sequence analyses of the cornea and of isolates from the contact lens case showed similarity with the T4 genotype. To the best of our knowledge, this is the first report of T4 Acanthamoeba keratitis case from the Midwest region of Brazil.
ISSN:0037-8682
1678-9849
1678-9849
DOI:10.1590/0037-8682-0010-2018