Myosin II isoforms play distinct roles in adherens junction biogenesis

Adherens junction (AJ) assembly under force is essential for many biological processes like epithelial monolayer bending, collective cell migration, cell extrusion and wound healing. The acto-myosin cytoskeleton acts as a major force-generator during the de novo formation and remodeling of AJ. Here,...

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Bibliographic Details
Published in:eLife 2019-09, Vol.8
Main Authors: Heuzé, Mélina L, Sankara Narayana, Gautham Hari Narayana, D'Alessandro, Joseph, Cellerin, Victor, Dang, Tien, Williams, David S, Van Hest, Jan Cm, Marcq, Philippe, Mège, René-Marc, Ladoux, Benoit
Format: Article
Language:English
Subjects:
Actin
adherens junctions
Adherens Junctions - metabolism
Animals
Anisotropy
Antigens, CD - metabolism
Biosynthesis
Cadherins - metabolism
Cell adhesion & migration
Cell Behavior
Cell Biology
Cell junctions
Cell Line
Cell migration
Cellular Biology
Cytoskeleton
Dogs
E-cadherin
epithelial cells
Epithelial Cells - metabolism
Humans
Isoforms
Life Sciences
mechanobiology
Microscopy
Morphogenesis
Muscle proteins
Myosin
Myosin Heavy Chains - metabolism
Nonmuscle Myosin Type IIB - metabolism
Observations
Physics of Living Systems
Physiological aspects
Protein Binding
Wound care
Wound healing
Wounds
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Summary:Adherens junction (AJ) assembly under force is essential for many biological processes like epithelial monolayer bending, collective cell migration, cell extrusion and wound healing. The acto-myosin cytoskeleton acts as a major force-generator during the de novo formation and remodeling of AJ. Here, we investigated the role of non-muscle myosin II isoforms (NMIIA and NMIIB) in epithelial junction assembly. NMIIA and NMIIB differentially regulate biogenesis of AJ through association with distinct actin networks. Analysis of junction dynamics, actin organization, and mechanical forces of control and knockdown cells for myosins revealed that NMIIA provides the mechanical tugging force necessary for cell-cell junction reinforcement and maintenance. NMIIB is involved in E-cadherin clustering, maintenance of a branched actin layer connecting E-cadherin complexes and perijunctional actin fibres leading to the building-up of anisotropic stress. These data reveal unanticipated complementary functions of NMIIA and NMIIB in the biogenesis and integrity of AJ.
ISSN:2050-084X
2050-084X
DOI:10.7554/eLife.46599