Comparative analysis of the carrot miRNAome in response to salt stress

• Published in: Scientific reports 2023-12, Vol.13 (1), p.21506-21506, Article 21506
• Main Authors: Szymonik, Kamil, Klimek-Chodacka, Magdalena, Lukasiewicz, Aneta, Macko-Podgórni, Alicja, Grzebelus, Dariusz, Baranski, Rafal
• Format: Article
• Language: English
• Subjects: 631/449; 631/449/2661; 631/449/2661/1797; Abiotic stress; Carrots; Cell walls; Chloroplasts; Comparative analysis; Cytotoxicity; Daucus carota - genetics; Daucus carota - metabolism; Gene Expression Regulation, Plant; Glutathione; Humanities and Social Sciences; MicroRNAs; MicroRNAs - genetics; MicroRNAs - metabolism; miRNA; Molecular modelling; multidisciplinary; Plant growth; Saline soils; Salinity; Salinity effects; Salinity tolerance; Salt; Salt tolerance; Salt Tolerance - genetics; Science; Science (multidisciplinary); Signal transduction; Soil; Soil salinity; Stress, Physiological - genetics; Vegetables; Water uptake
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-023-48900-0

Soil salinity adversely affects the yield and quality of crops, including carrot. During salt stress, plant growth and development are impaired by restricted water uptake and ion cytotoxicity, leading to nutrient imbalance and oxidative burst. However, the molecular mechanisms of the carrot plant response to salt stress remain unclear. The occurrence and expression of miRNAs that are potentially involved in the regulation of carrot tolerance to salinity stress were investigated. The results of small RNA sequencing revealed that salt-sensitive (DH1) and salt-tolerant (DLBA) carrot varieties had different miRNA expression profiles. A total of 95 miRNAs were identified, including 71 novel miRNAs, of which 30 and 23 were unique to DH1 and DLBA, respectively. The comparison of NGS and qPCR results allowed identification of two conserved and five novel miRNA involved in carrot response to salt stress, and which differentiated the salt-tolerant and salt-sensitive varieties. Degradome analysis supported by in silico-based predictions and followed by expression analysis of exemplary target genes pointed at genes related to proline, glutathione, and glutamate metabolism pathways as potential miRNA targets involved in salt tolerance, and indicated that the regulation of osmoprotection and antioxidant protection, earlier identified as being more efficient in the tolerant variety, may be controlled by miRNAs. Furthermore, potential miRNA target genes involved in chloroplast protection, signal transduction and the synthesis and modification of cell wall components were indicated in plants growing in saline soil.