Protective Properties of S-layer Protein 2 from Lactobacillus crispatus 2029 against Candida albicans Infections

• Published in: Biomolecules (Basel, Switzerland) Switzerland), 2023-12, Vol.13 (12), p.1740
• Main Authors: Abramov, Vyacheslav M, Kosarev, Igor V, Machulin, Andrey V, Priputnevich, Tatiana V, Deryusheva, Evgenia I, Panin, Alexander N, Chikileva, Irina O, Abashina, Tatiana N, Melnikov, Vyacheslav G, Suzina, Nataliya E, Nikonov, Ilia N, Akhmetzyanova, Anna A, Khlebnikov, Valentin S, Sakulin, Vadim K, Vasilenko, Raisa N, Samoilenko, Vladimir A, Gordeev, Alexey B, Sukhikh, Gennady T, Uversky, Vladimir N, Karlyshev, Andrey V
• Format: Article
• Language: English
• Subjects: Acquired immune deficiency syndrome; Adaptation; Adhesion; Adhesives; AIDS; anti-adhesive effect; anti-pathogenic potential; Antibiotics; Biofilms; Campylobacter; Candida albicans; Clinical isolates; Colonization; Cytokines; Diabetes; Drug resistance; Epithelial cells; Foodborne diseases; Foodborne pathogens; Fungal infections; Genomes; HeLa cells; Inflammation; Lactobacillus crispatus; Microbiota; Microorganisms; Morbidity; Mortality; Pathogens; probiotic bacteria; Probiotics; Proteins; S-layer protein 2; Strains (organisms); Toxicity; Tropical diseases; Vagina; Viral infections; Womens health
• ISSN: 2218-273X; 2218-273X
• DOI: 10.3390/biom13121740

Previously, the protective role of the S-layer protein 2 (Slp2) of the vaginal 2029 (LC2029) strain against foodborne pathogens , serovar Enteritidis, and O157:H was demonstrated. We demonstrate the new roles of the Slp2-positive LC2029 strain and soluble Slp2 against infections. We show that LC2029 bacteria can adhere to the surface of the cervical epithelial HeLa cells, prevent their contact with , and block yeast transition to a pathogenic hyphal form. Surface-bound Slp2 provides the ability for LC2029 to co-aggregate with various strains, including clinical isolates. -induced necrotizing epithelial damage is reduced by colonization with the Slp2-positive LC2029 strain. Slp2 inhibits the adhesion of various strains of to different human epithelial cells, blocks yeast transition to a pathogenic hyphal form, and prevents the colonization and pathogenic infiltration of mucosal barriers. Only Slp2 and LC2029 bacteria stimulate the production of protective human β-defensin 3 in various epithelial cells. These findings support the anti- potential of the probiotic LC2029 strain and Slp2 and form the basis for further research on their ability to prevent and manage invasive infections.