A Gold Growth-Based Plasmonic ELISA for the Sensitive Detection of Fumonisin B1 in Maize

In this paper, a highly sensitive plasmonic enzyme-linked immunosorbent assay (pELISA) was developed for the naked-eye detection of fumonisin B1 (FB1). Glucose oxidase (GOx) was used as an alternative to horseradish peroxidase as the carrier of the competing antigen. GOx catalyzed the oxidation of g...

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Bibliographic Details
Published in:Toxins 2019-06, Vol.11 (6), p.323
Main Authors: Zhan, Shengnan, Zheng, Lingyan, Zhou, Yaofeng, Wu, Kesheng, Duan, Hong, Huang, Xiaolin, Xiong, Yonghua
Format: Article
Language:English
Subjects:
Antigens
Catalytic oxidation
Chemical pollution
Contaminants
controlled growth kinetics
Corn
Enzyme-linked immunosorbent assay
Enzymes
Extinction
Fumonisin B1
fumonisins B1
Glucose
Glucose oxidase
Gold
Horseradish peroxidase
Hydrogen peroxide
Mass spectrometry
Morphology
naked-eye detection
Oxidation
Peroxidase
plasmonic enzyme-linked immunosorbent assay
Plasmonics
Reducing agents
Scientific imaging
Seeds
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Summary:In this paper, a highly sensitive plasmonic enzyme-linked immunosorbent assay (pELISA) was developed for the naked-eye detection of fumonisin B1 (FB1). Glucose oxidase (GOx) was used as an alternative to horseradish peroxidase as the carrier of the competing antigen. GOx catalyzed the oxidation of glucose to produce hydrogen peroxide, which acted as a reducing agent to reduce Au3+ to Au on the surface of gold seeds (5 nm), This reaction led to a color change in the solution from colorless to purple, which was observable to the naked eye. Various parameters that could influence the detection performance of pELISA were investigated. The developed method exhibited a considerably high sensitivity for FB1 qualitative naked-eye detection, with a visible cut-off limit of 1.25 ng/mL. Moreover, the proposed pELISA showed a good linear range of 0.31–10 ng/mL with a half maximal inhibitory concentration (IC50) of 1.86 ng/mL, which was approximately 13-fold lower than that of a horseradish peroxidase- (HRP)-based conventional ELISA. Meanwhile, the proposed method was highly specific and accurate. In summary, the new pELISA exhibited acceptable accuracy and precision for sensitive naked-eye detection of FB1 in maize samples and can be applied for the detection of other chemical contaminants.
ISSN:2072-6651
2072-6651
DOI:10.3390/toxins11060323